Design and Analysis of Single-Cell Sequencing Experiments

Research output: Contribution to journal/periodicalArticleScientificpeer-review

351 Citations (Scopus)


Recent advances in single-cell sequencing hold great potential for exploring biological systems with unprecedented resolution. Sequencing the genome of individual cells can reveal somatic mutations and allows the investigation of clonal dynamics. Single-cell transcriptome sequencing can elucidate the cell type composition of a sample. However, single-cell sequencing comes with major technical challenges and yields complex data output. In this Primer, we provide an overview of available methods and discuss experimental design and single-cell data analysis. We hope that these guidelines will enable a growing number of researchers to leverage the power of single-cell sequencing.

Original languageEnglish
Pages (from-to)799-810
Number of pages12
Issue number4
Publication statusPublished - 05 Nov 2015


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