Design and Analysis of Single-Cell Sequencing Experiments

TY - JOUR

T1 - Design and Analysis of Single-Cell Sequencing Experiments

AU - Grün, Dominic

AU - van Oudenaarden, Alexander

N1 - Copyright © 2015 Elsevier Inc. All rights reserved.

PY - 2015/11/5

Y1 - 2015/11/5

N2 - Recent advances in single-cell sequencing hold great potential for exploring biological systems with unprecedented resolution. Sequencing the genome of individual cells can reveal somatic mutations and allows the investigation of clonal dynamics. Single-cell transcriptome sequencing can elucidate the cell type composition of a sample. However, single-cell sequencing comes with major technical challenges and yields complex data output. In this Primer, we provide an overview of available methods and discuss experimental design and single-cell data analysis. We hope that these guidelines will enable a growing number of researchers to leverage the power of single-cell sequencing.

AB - Recent advances in single-cell sequencing hold great potential for exploring biological systems with unprecedented resolution. Sequencing the genome of individual cells can reveal somatic mutations and allows the investigation of clonal dynamics. Single-cell transcriptome sequencing can elucidate the cell type composition of a sample. However, single-cell sequencing comes with major technical challenges and yields complex data output. In this Primer, we provide an overview of available methods and discuss experimental design and single-cell data analysis. We hope that these guidelines will enable a growing number of researchers to leverage the power of single-cell sequencing.

U2 - 10.1016/j.cell.2015.10.039

DO - 10.1016/j.cell.2015.10.039

M3 - Article

C2 - 26544934

SN - 0092-8674

VL - 163

SP - 799

EP - 810

JO - Cell

JF - Cell

IS - 4

ER -