Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections

Research output: Contribution to journal/periodicalArticleScientificpeer-review


The use of tagged RNA probes to directly hybridize frozen sections of chemically fixed tissues, followed by the tag detection with specific antibodies and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous gurken and bicoid mRNAs in Drosophila oocytes.

Original languageEnglish
Pages (from-to)177-186
Number of pages10
JournalMethods in Molecular Biology
Publication statusPublished - 2018


  • Animals
  • Cryoultramicrotomy
  • Drosophila melanogaster/genetics
  • Female
  • Frozen Sections
  • In Situ Hybridization/methods
  • Microscopy, Immunoelectron/methods
  • Paraffin Embedding
  • RNA, Messenger/genetics
  • Tissue Fixation


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