The use of tagged RNA probes to directly hybridize frozen sections of chemically fixed tissues, followed by the tag detection with specific antibodies and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous gurken and bicoid mRNAs in Drosophila oocytes.
- Drosophila melanogaster/genetics
- Frozen Sections
- In Situ Hybridization/methods
- Microscopy, Immunoelectron/methods
- Paraffin Embedding
- RNA, Messenger/genetics
- Tissue Fixation