Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections

TY - JOUR

T1 - Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections

AU - Rabouille, Catherine

PY - 2018

Y1 - 2018

N2 - The use of tagged RNA probes to directly hybridize frozen sections of chemically fixed tissues, followed by the tag detection with specific antibodies and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous gurken and bicoid mRNAs in Drosophila oocytes.

AB - The use of tagged RNA probes to directly hybridize frozen sections of chemically fixed tissues, followed by the tag detection with specific antibodies and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous gurken and bicoid mRNAs in Drosophila oocytes.

KW - Animals

KW - Cryoultramicrotomy

KW - Drosophila melanogaster/genetics

KW - Female

KW - Frozen Sections

KW - In Situ Hybridization/methods

KW - Microscopy, Immunoelectron/methods

KW - Paraffin Embedding

KW - RNA, Messenger/genetics

KW - Tissue Fixation

U2 - 10.1007/978-1-4939-7213-5_11

DO - 10.1007/978-1-4939-7213-5_11

M3 - Article

C2 - 29130197

SN - 1064-3745

VL - 1649

SP - 177

EP - 186

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -