Determining long-range chromatin interactions for selected genomic sites using 4C-seq technology: From fixation to computation

E. Splinter, E. de Wit, H.J. van de Werken, P. Klous, W. de Laat

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Chromosome Conformation Capture (3C) and 3C-based technologies are constantly evolving in order to probe nuclear organization with higher depth and resolution. One such method is 4C-technology that allows the investigation of the nuclear environment of a locus of choice. The use of Illumina next generation sequencing as a detection platform for the analysis of 4C data has further improved the sensitivity and resolution of this method. Here we provide a step-by-step protocol for 4C-seq, describing the procedure from the initial template preparation until the final data analysis, interchanged with background information and considerations.
Original languageEnglish
Pages (from-to)221-230
JournalMethods
Volume58
Issue number3
DOIs
Publication statusPublished - 2012

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