Differential Runx3, Eomes, and T-bet expression subdivides MS-associated CD4+ T cells with brain-homing capacity

Cindy Hoeks, Fabiënne van Puijfelik, Steven C Koetzier, Jasper Rip, Cato E A Corsten, Annet F Wierenga-Wolf, Marie-José Melief, Piet Stinissen, Joost Smolders, Niels Hellings, Bieke Broux, Marvin M van Luijn

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Abstract

Multiple sclerosis (MS) is a common and devastating chronic inflammatory disease of the central nervous system (CNS). CD4+ T cells are assumed to be the first to cross the blood-CNS barrier and trigger local inflammation. Here, we explored how pathogenicity-associated effector programs define CD4+ T cell subsets with brain-homing ability in MS. Runx3- and Eomes-, but not T-bet-expressing CD4+ memory cells were diminished in the blood of MS patients. This decline reversed following natalizumab treatment and was supported by an Runx3+ Eomes+ T-bet- enrichment in cerebrospinal fluid samples of treatment-naïve MS patients. This transcription factor profile was associated with high granzyme K (GZMK) and CCR5 levels and was most prominent in Th17.1 cells (CCR6+ CXCR3+ CCR4-/dim ). Previously published CD28- CD4 T cells were characterized by a Runx3+ Eomes- T-bet+ phenotype that coincided with intermediate CCR5 and a higher granzyme B (GZMB) and perforin expression, indicating the presence of two separate subsets. Under steady-state conditions, granzyme Khigh Th17.1 cells spontaneously passed the blood-brain barrier in vitro. This was only found for other subsets including CD28- cells when using inflamed barriers. Altogether, CD4+ T cells contain small fractions with separate pathogenic features, of which Th17.1 seems to breach the blood-brain barrier as a possible early event in MS. This article is protected by copyright. All rights reserved.

Original languageEnglish
Pages (from-to)e2350544
JournalEuropean Journal of Immunology
Volume54
DOIs
Publication statusPublished - Jan 2024

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