Directed Differentiation of Murine and Human Small Intestinal Organoids Toward All Mature Lineages

A Martinez-Silgado, J Beumer, H Clevers

Research output: Contribution to journal/periodicalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Intestinal organoids are three-dimensional structures derived from tissue-resident adult stem cells. These organoids recapitulate key aspects of epithelial biology and can be used to study homeostatic turnover of the corresponding tissue. Organoids can be enriched for the various mature lineages which allows studies of the respective differentiation processes and of the diverse cellular functions. Here we describe mechanisms of intestinal fate specification and how these can be exploited to drive mouse and human small intestinal organoids into each of the functionally mature lineages.

Original languageEnglish
Pages (from-to)107-122
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume2650
DOIs
Publication statusPublished - 2023

Keywords

  • Adult
  • Humans
  • Animals
  • Mice
  • Intestine, Small
  • Cell Differentiation
  • Adult Stem Cells
  • Homeostasis
  • Organoids

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