Standard

Divergent members of the bacterial division Verrucomicrobiales in a temperate freshwater lake. / Zwart, G.; Huismans, R.; Van Agterveld, M.P.; Van de Peer, Y.; De Rijk, P.; Eenhoorn, H.; Muyzer, G.; Van Hannen, E.J.; Gons, H.J.; Laanbroek, H.J.

In: FEMS Microbiology Ecology, Vol. 25, No. 2, 1998, p. 159-169.

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Harvard

Zwart, G, Huismans, R, Van Agterveld, MP, Van de Peer, Y, De Rijk, P, Eenhoorn, H, Muyzer, G, Van Hannen, EJ, Gons, HJ & Laanbroek, HJ 1998, 'Divergent members of the bacterial division Verrucomicrobiales in a temperate freshwater lake' FEMS Microbiology Ecology, vol. 25, no. 2, pp. 159-169. https://doi.org/10.1016/S0168-6496(97)00092-5

APA

Zwart, G., Huismans, R., Van Agterveld, M. P., Van de Peer, Y., De Rijk, P., Eenhoorn, H., ... Laanbroek, H. J. (1998). Divergent members of the bacterial division Verrucomicrobiales in a temperate freshwater lake. FEMS Microbiology Ecology, 25(2), 159-169. https://doi.org/10.1016/S0168-6496(97)00092-5

Vancouver

Zwart G, Huismans R, Van Agterveld MP, Van de Peer Y, De Rijk P, Eenhoorn H et al. Divergent members of the bacterial division Verrucomicrobiales in a temperate freshwater lake. FEMS Microbiology Ecology. 1998;25(2):159-169. https://doi.org/10.1016/S0168-6496(97)00092-5

Author

Zwart, G. ; Huismans, R. ; Van Agterveld, M.P. ; Van de Peer, Y. ; De Rijk, P. ; Eenhoorn, H. ; Muyzer, G. ; Van Hannen, E.J. ; Gons, H.J. ; Laanbroek, H.J. / Divergent members of the bacterial division Verrucomicrobiales in a temperate freshwater lake. In: FEMS Microbiology Ecology. 1998 ; Vol. 25, No. 2. pp. 159-169.

BibTeX

@article{755fa54f75b1431d8f2763b8f4ddc50e,
title = "Divergent members of the bacterial division Verrucomicrobiales in a temperate freshwater lake",
abstract = "Bacterial diversity in the water column of a freshwater lake in the Netherlands was investigated by analysis of 16S rRNA gene sequences recovered through PCR amplification from total community DNA. Among 23 unique cloned sequences, two appeared to belong to the recently described bacterial division Verrucomicrobiales. One of the two sequences was most similar to a group of environmental clones that form a distinct lineage within the division. The other sequence was divergent (less than 85{\%} similarity) from all 16S rRNA gene sequences, both from cultivated species and from environmental clones, known in this division to date. Analysis by denaturing gradient gel electrophoresis (DGGE) and sequencing of DNA recovered through excision from the DGGE gel showed that the two sequence types were present in the lake throughout the year. [KEYWORDS: bacterial diversity; freshwater pelagic zone; denaturing gradient gel electrophoresis; phylogeny; 16S rRNA;Verrucomicrobium; Prosthecobacter 16s ribosomal-rna; polymerase chain-reaction; gene amplification; pcr; sequences; community; diversity; alignment; tool]",
author = "G. Zwart and R. Huismans and {Van Agterveld}, M.P. and {Van de Peer}, Y. and {De Rijk}, P. and H. Eenhoorn and G. Muyzer and {Van Hannen}, E.J. and H.J. Gons and H.J. Laanbroek",
note = "Reporting year: 1998 Metis note: 2291; CL; MWE ; ME file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs1998/Zwart_ea_2291.pdf",
year = "1998",
doi = "10.1016/S0168-6496(97)00092-5",
language = "English",
volume = "25",
pages = "159--169",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Divergent members of the bacterial division Verrucomicrobiales in a temperate freshwater lake

AU - Zwart, G.

AU - Huismans, R.

AU - Van Agterveld, M.P.

AU - Van de Peer, Y.

AU - De Rijk, P.

AU - Eenhoorn, H.

AU - Muyzer, G.

AU - Van Hannen, E.J.

AU - Gons, H.J.

AU - Laanbroek, H.J.

N1 - Reporting year: 1998 Metis note: 2291; CL; MWE ; ME file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs1998/Zwart_ea_2291.pdf

PY - 1998

Y1 - 1998

N2 - Bacterial diversity in the water column of a freshwater lake in the Netherlands was investigated by analysis of 16S rRNA gene sequences recovered through PCR amplification from total community DNA. Among 23 unique cloned sequences, two appeared to belong to the recently described bacterial division Verrucomicrobiales. One of the two sequences was most similar to a group of environmental clones that form a distinct lineage within the division. The other sequence was divergent (less than 85% similarity) from all 16S rRNA gene sequences, both from cultivated species and from environmental clones, known in this division to date. Analysis by denaturing gradient gel electrophoresis (DGGE) and sequencing of DNA recovered through excision from the DGGE gel showed that the two sequence types were present in the lake throughout the year. [KEYWORDS: bacterial diversity; freshwater pelagic zone; denaturing gradient gel electrophoresis; phylogeny; 16S rRNA;Verrucomicrobium; Prosthecobacter 16s ribosomal-rna; polymerase chain-reaction; gene amplification; pcr; sequences; community; diversity; alignment; tool]

AB - Bacterial diversity in the water column of a freshwater lake in the Netherlands was investigated by analysis of 16S rRNA gene sequences recovered through PCR amplification from total community DNA. Among 23 unique cloned sequences, two appeared to belong to the recently described bacterial division Verrucomicrobiales. One of the two sequences was most similar to a group of environmental clones that form a distinct lineage within the division. The other sequence was divergent (less than 85% similarity) from all 16S rRNA gene sequences, both from cultivated species and from environmental clones, known in this division to date. Analysis by denaturing gradient gel electrophoresis (DGGE) and sequencing of DNA recovered through excision from the DGGE gel showed that the two sequence types were present in the lake throughout the year. [KEYWORDS: bacterial diversity; freshwater pelagic zone; denaturing gradient gel electrophoresis; phylogeny; 16S rRNA;Verrucomicrobium; Prosthecobacter 16s ribosomal-rna; polymerase chain-reaction; gene amplification; pcr; sequences; community; diversity; alignment; tool]

U2 - 10.1016/S0168-6496(97)00092-5

DO - 10.1016/S0168-6496(97)00092-5

M3 - Article

VL - 25

SP - 159

EP - 169

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 2

ER -

ID: 339413