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Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction. / Riedl, J.A.; Brandt, D.T.; Batlle, E.; Price, L.S.; Clevers, J.C.; Bos, J.L.

In: The Biochemical journal, Vol. 389, 2005, p. 465-469.

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Harvard

Riedl, JA, Brandt, DT, Batlle, E, Price, LS, Clevers, JC & Bos, JL 2005, 'Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction.' The Biochemical journal, vol. 389, pp. 465-469.

APA

Riedl, J. A., Brandt, D. T., Batlle, E., Price, L. S., Clevers, J. C., & Bos, J. L. (2005). Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction. The Biochemical journal, 389, 465-469.

Vancouver

Riedl JA, Brandt DT, Batlle E, Price LS, Clevers JC, Bos JL. Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction. The Biochemical journal. 2005;389:465-469.

Author

Riedl, J.A. ; Brandt, D.T. ; Batlle, E. ; Price, L.S. ; Clevers, J.C. ; Bos, J.L./ Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction.In: The Biochemical journal. 2005 ; Vol. 389. pp. 465-469

BibTeX

@article{684365b5492449ca991246d6f9ef6edf,
title = "Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction.",
abstract = "Ephrins are cell surface ligands that activate Eph receptor tyrosine kinases. This ligand-receptor interaction plays a central role in the sorting of cells. We have previously shown that the ephrinB-EphB signalling pathway is also involved in the migration of intestinal precursor cells along the crypts. Using the colon cell line DLD1 expressing the EphB2 receptor, we showed that stimulation of these cells with soluble ephrinB1 results in a rapid retraction of cell extensions and a detachment of cells. On ephrinB1 stimulation, the small GTPases Rho and Ras are activated and Rap1 is inactivated. Importantly, when a constitutively active Rap1 mutant was introduced into these cells, ephrinB1-induced retraction was inhibited. From these results, we conclude that down-regulation of Rap1 is a prerequisite for ephrin-induced cell retraction in colon cells.",
author = "J.A. Riedl and D.T. Brandt and E. Batlle and L.S. Price and J.C. Clevers and J.L. Bos",
note = "doi: 10.1042/BJ20050048. PMC_URL: http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=pmcentrez&artid=1175124&blobtype=pdf",
year = "2005",
language = "English",
volume = "389",
pages = "465--469",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",

}

RIS

TY - JOUR

T1 - Down-regulation of Rap1 activity is involved in ephrinB1-induced cell contraction.

AU - Riedl,J.A.

AU - Brandt,D.T.

AU - Batlle,E.

AU - Price,L.S.

AU - Clevers,J.C.

AU - Bos,J.L.

N1 - doi: 10.1042/BJ20050048. PMC_URL: http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=pmcentrez&artid=1175124&blobtype=pdf

PY - 2005

Y1 - 2005

N2 - Ephrins are cell surface ligands that activate Eph receptor tyrosine kinases. This ligand-receptor interaction plays a central role in the sorting of cells. We have previously shown that the ephrinB-EphB signalling pathway is also involved in the migration of intestinal precursor cells along the crypts. Using the colon cell line DLD1 expressing the EphB2 receptor, we showed that stimulation of these cells with soluble ephrinB1 results in a rapid retraction of cell extensions and a detachment of cells. On ephrinB1 stimulation, the small GTPases Rho and Ras are activated and Rap1 is inactivated. Importantly, when a constitutively active Rap1 mutant was introduced into these cells, ephrinB1-induced retraction was inhibited. From these results, we conclude that down-regulation of Rap1 is a prerequisite for ephrin-induced cell retraction in colon cells.

AB - Ephrins are cell surface ligands that activate Eph receptor tyrosine kinases. This ligand-receptor interaction plays a central role in the sorting of cells. We have previously shown that the ephrinB-EphB signalling pathway is also involved in the migration of intestinal precursor cells along the crypts. Using the colon cell line DLD1 expressing the EphB2 receptor, we showed that stimulation of these cells with soluble ephrinB1 results in a rapid retraction of cell extensions and a detachment of cells. On ephrinB1 stimulation, the small GTPases Rho and Ras are activated and Rap1 is inactivated. Importantly, when a constitutively active Rap1 mutant was introduced into these cells, ephrinB1-induced retraction was inhibited. From these results, we conclude that down-regulation of Rap1 is a prerequisite for ephrin-induced cell retraction in colon cells.

M3 - Article

VL - 389

SP - 465

EP - 469

JO - Biochemical Journal

T2 - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

ER -

ID: 424748