Effect of model root exudate on denitrifier community dynamics and activity at different water-filled pore space levels in a fertilised soil

TY - JOUR

T1 - Effect of model root exudate on denitrifier community dynamics and activity at different water-filled pore space levels in a fertilised soil

AU - Langarica-Fuentes, A.

AU - Manrubia, Marta

AU - Giles, M.E.

AU - Mitchell, S.

AU - Daniell, T.J.

N1 - 6476, TE;

PY - 2018

Y1 - 2018

N2 - Although a "rhizosphere effect" on denitrification rates has been established, a clear understanding of the effects of exudate addition on denitrifier community dynamics remains elusive. A microcosm experiment was designed to explore the interaction between exudate addition and soil moisture on community dynamics and denitrification rates. Artificial root exudate at 5 different carbon concentrations was added daily to soil microcosms at contrasting target WFPS (50, 70 and 90. After a 7-day period, total denitrification and N2O emission rates were measured and community dynamics assessed using molecular methods. The response of denitrifier genes to exudate addition was different, with nirS and nosZ-I showing a stronger effect than nirK and nosZ-II. Distinct community structures were observed for nirS and nosZ-I at 90% target WFPS when compared to 50% and 70 NirS denitrifier population size showed a ca. 5-fold increase in gene copy number at 90% WFPS when exudate was added at the highest C input. Significant total denitrification and N2O emission rates were observed only at 90% WFPS, which increased with C input. Our study improves the understanding of the complex interaction between microbial communities, the abiotic environment and process rates which can inform management practices aimed at increasing complete denitrification and controlling greenhouse gas production from agriculture.

AB - Although a "rhizosphere effect" on denitrification rates has been established, a clear understanding of the effects of exudate addition on denitrifier community dynamics remains elusive. A microcosm experiment was designed to explore the interaction between exudate addition and soil moisture on community dynamics and denitrification rates. Artificial root exudate at 5 different carbon concentrations was added daily to soil microcosms at contrasting target WFPS (50, 70 and 90. After a 7-day period, total denitrification and N2O emission rates were measured and community dynamics assessed using molecular methods. The response of denitrifier genes to exudate addition was different, with nirS and nosZ-I showing a stronger effect than nirK and nosZ-II. Distinct community structures were observed for nirS and nosZ-I at 90% target WFPS when compared to 50% and 70 NirS denitrifier population size showed a ca. 5-fold increase in gene copy number at 90% WFPS when exudate was added at the highest C input. Significant total denitrification and N2O emission rates were observed only at 90% WFPS, which increased with C input. Our study improves the understanding of the complex interaction between microbial communities, the abiotic environment and process rates which can inform management practices aimed at increasing complete denitrification and controlling greenhouse gas production from agriculture.

KW - Denitrification

KW - root exudate addition

KW - microbial community dynamics

KW - nitrous 20 oxide emissions

KW - nirK

KW - nirS

KW - nosZ

KW - international

U2 - 10.1016/j.soilbio.2018.01.034

DO - 10.1016/j.soilbio.2018.01.034

M3 - Article

SN - 0038-0717

VL - 120

SP - 70

EP - 79

JO - Soil Biology & Biochemistry

JF - Soil Biology & Biochemistry

IS - May

ER -