Effects of probiotic consortia on plant metabolites are associated with soil indigenous microbiota and fertilization regimes

Zhikang Wang; Ziyun Chen; Marcio F.A. Leite; Ziheng Xu; Quan Lin; George A. Kowalchuk; Xiangxiang Fu; Eiko E. Kuramae

doi:10.1016/j.indcrop.2022.115138

Effects of probiotic consortia on plant metabolites are associated with soil indigenous microbiota and fertilization regimes

Zhikang Wang, Ziyun Chen, Marcio F.A. Leite, Ziheng Xu, Quan Lin, George A. Kowalchuk, Xiangxiang Fu^* (Corresponding author), Eiko E. Kuramae

^*Corresponding author for this work

NIOO - Microbial Ecology (ME)

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

12 Citations (Scopus)

38 Downloads (Pure)

Abstract

Introducing probiotics to soil is a sustainable way to stimulate the production of plant metabolites. However, the soil-resident microbes may compromise the efficiency of probiotics. To date, it remains challenging to integrate the effects of probiotics on plant performance with soil microbiome changes. Using Cyclocarya paliurus (Batal.) Iljinsk as a model medicinal plant and two types of probiotic consortia combined with organic fertilizer at three levels (low: 0.5, medium: 1.0, and high: 1.5 kg·plant⁻¹), we examined the impacts of three fertilization regimes (O: organic fertilizer, OMF: O coupled with Bacillus megaterium and Pseudomonas fluorescens, OCB: O coupled with Azotobacter chroococcum and Azospirillum brasilense) on plant metabolites and nutrient stoichiometry after three-year applications and identified the key soil microbes relating to the accumulation of plant metabolites via generalized joint attribute model (GJAM) analysis. Our results indicated that the concentration of flavonoids reached 36.9 mg·g⁻¹ in OCB treatment at a low level, and 30.0 mg·g⁻¹ in OMF treatment at a medium level, both were significantly higher than that in O treatment (25.8 mg·g⁻¹ on average). Furthermore, the accumulations of metabolites were associated with plant nutrient acquisition and C: N: P stoichiometry. GJAM analysis showed that higher fertilizer levels restricted the influence of probiotic consortia on the variance of plant-soil-microbe system, with fewer differences observed between fertilizer types. Specific soil microbes were predicted as potential indicators that may assist or impede the effects of probiotics on plant metabolite production. The predictions were further tested in a comparative pot experiment, and the effects of common indicators in both pot and field experiments were consistently associated with probiotics’ addition. This study reveals that the effects of probiotics on plant metabolites are associated with fertilization regimes and soil-indigenous microbes. Identifying microbial indicators will help to understand the probiotics' effects and further improve plant productivity.

Original language	English
Article number	115138
Journal	Industrial Crops and Products
Volume	185
DOIs	https://doi.org/10.1016/j.indcrop.2022.115138
Publication status	Published - 01 Oct 2022

Keywords

Generalized joint attribute model
Microbial indicator
Plant metabolites
Plant probiotics
Soil microbiome

Access to Document

10.1016/j.indcrop.2022.115138

Wang, Z. et al 2022Final published version, 5.4 MB

Cite this

@article{eff22d9947424897ae0a76c8c98d5d25,

title = "Effects of probiotic consortia on plant metabolites are associated with soil indigenous microbiota and fertilization regimes",

abstract = "Introducing probiotics to soil is a sustainable way to stimulate the production of plant metabolites. However, the soil-resident microbes may compromise the efficiency of probiotics. To date, it remains challenging to integrate the effects of probiotics on plant performance with soil microbiome changes. Using Cyclocarya paliurus (Batal.) Iljinsk as a model medicinal plant and two types of probiotic consortia combined with organic fertilizer at three levels (low: 0.5, medium: 1.0, and high: 1.5 kg·plant−1), we examined the impacts of three fertilization regimes (O: organic fertilizer, OMF: O coupled with Bacillus megaterium and Pseudomonas fluorescens, OCB: O coupled with Azotobacter chroococcum and Azospirillum brasilense) on plant metabolites and nutrient stoichiometry after three-year applications and identified the key soil microbes relating to the accumulation of plant metabolites via generalized joint attribute model (GJAM) analysis. Our results indicated that the concentration of flavonoids reached 36.9 mg·g−1 in OCB treatment at a low level, and 30.0 mg·g−1 in OMF treatment at a medium level, both were significantly higher than that in O treatment (25.8 mg·g−1 on average). Furthermore, the accumulations of metabolites were associated with plant nutrient acquisition and C: N: P stoichiometry. GJAM analysis showed that higher fertilizer levels restricted the influence of probiotic consortia on the variance of plant-soil-microbe system, with fewer differences observed between fertilizer types. Specific soil microbes were predicted as potential indicators that may assist or impede the effects of probiotics on plant metabolite production. The predictions were further tested in a comparative pot experiment, and the effects of common indicators in both pot and field experiments were consistently associated with probiotics{\textquoteright} addition. This study reveals that the effects of probiotics on plant metabolites are associated with fertilization regimes and soil-indigenous microbes. Identifying microbial indicators will help to understand the probiotics' effects and further improve plant productivity.",

keywords = "Generalized joint attribute model, Microbial indicator, Plant metabolites, Plant probiotics, Soil microbiome",

author = "Zhikang Wang and Ziyun Chen and Leite, {Marcio F.A.} and Ziheng Xu and Quan Lin and Kowalchuk, {George A.} and Xiangxiang Fu and Kuramae, {Eiko E.}",

note = "7470, ME",

year = "2022",

month = oct,

day = "1",

doi = "10.1016/j.indcrop.2022.115138",

language = "English",

volume = "185",

journal = "Industrial Crops and Products",

issn = "0926-6690",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Effects of probiotic consortia on plant metabolites are associated with soil indigenous microbiota and fertilization regimes

AU - Wang, Zhikang

AU - Chen, Ziyun

AU - Leite, Marcio F.A.

AU - Xu, Ziheng

AU - Lin, Quan

AU - Kowalchuk, George A.

AU - Fu, Xiangxiang

AU - Kuramae, Eiko E.

N1 - 7470, ME

PY - 2022/10/1

Y1 - 2022/10/1

N2 - Introducing probiotics to soil is a sustainable way to stimulate the production of plant metabolites. However, the soil-resident microbes may compromise the efficiency of probiotics. To date, it remains challenging to integrate the effects of probiotics on plant performance with soil microbiome changes. Using Cyclocarya paliurus (Batal.) Iljinsk as a model medicinal plant and two types of probiotic consortia combined with organic fertilizer at three levels (low: 0.5, medium: 1.0, and high: 1.5 kg·plant−1), we examined the impacts of three fertilization regimes (O: organic fertilizer, OMF: O coupled with Bacillus megaterium and Pseudomonas fluorescens, OCB: O coupled with Azotobacter chroococcum and Azospirillum brasilense) on plant metabolites and nutrient stoichiometry after three-year applications and identified the key soil microbes relating to the accumulation of plant metabolites via generalized joint attribute model (GJAM) analysis. Our results indicated that the concentration of flavonoids reached 36.9 mg·g−1 in OCB treatment at a low level, and 30.0 mg·g−1 in OMF treatment at a medium level, both were significantly higher than that in O treatment (25.8 mg·g−1 on average). Furthermore, the accumulations of metabolites were associated with plant nutrient acquisition and C: N: P stoichiometry. GJAM analysis showed that higher fertilizer levels restricted the influence of probiotic consortia on the variance of plant-soil-microbe system, with fewer differences observed between fertilizer types. Specific soil microbes were predicted as potential indicators that may assist or impede the effects of probiotics on plant metabolite production. The predictions were further tested in a comparative pot experiment, and the effects of common indicators in both pot and field experiments were consistently associated with probiotics’ addition. This study reveals that the effects of probiotics on plant metabolites are associated with fertilization regimes and soil-indigenous microbes. Identifying microbial indicators will help to understand the probiotics' effects and further improve plant productivity.

AB - Introducing probiotics to soil is a sustainable way to stimulate the production of plant metabolites. However, the soil-resident microbes may compromise the efficiency of probiotics. To date, it remains challenging to integrate the effects of probiotics on plant performance with soil microbiome changes. Using Cyclocarya paliurus (Batal.) Iljinsk as a model medicinal plant and two types of probiotic consortia combined with organic fertilizer at three levels (low: 0.5, medium: 1.0, and high: 1.5 kg·plant−1), we examined the impacts of three fertilization regimes (O: organic fertilizer, OMF: O coupled with Bacillus megaterium and Pseudomonas fluorescens, OCB: O coupled with Azotobacter chroococcum and Azospirillum brasilense) on plant metabolites and nutrient stoichiometry after three-year applications and identified the key soil microbes relating to the accumulation of plant metabolites via generalized joint attribute model (GJAM) analysis. Our results indicated that the concentration of flavonoids reached 36.9 mg·g−1 in OCB treatment at a low level, and 30.0 mg·g−1 in OMF treatment at a medium level, both were significantly higher than that in O treatment (25.8 mg·g−1 on average). Furthermore, the accumulations of metabolites were associated with plant nutrient acquisition and C: N: P stoichiometry. GJAM analysis showed that higher fertilizer levels restricted the influence of probiotic consortia on the variance of plant-soil-microbe system, with fewer differences observed between fertilizer types. Specific soil microbes were predicted as potential indicators that may assist or impede the effects of probiotics on plant metabolite production. The predictions were further tested in a comparative pot experiment, and the effects of common indicators in both pot and field experiments were consistently associated with probiotics’ addition. This study reveals that the effects of probiotics on plant metabolites are associated with fertilization regimes and soil-indigenous microbes. Identifying microbial indicators will help to understand the probiotics' effects and further improve plant productivity.

KW - Generalized joint attribute model

KW - Microbial indicator

KW - Plant metabolites

KW - Plant probiotics

KW - Soil microbiome

U2 - 10.1016/j.indcrop.2022.115138

DO - 10.1016/j.indcrop.2022.115138

M3 - Article

AN - SCOPUS:85131414489

SN - 0926-6690

VL - 185

JO - Industrial Crops and Products

JF - Industrial Crops and Products

M1 - 115138

ER -

Effects of probiotic consortia on plant metabolites are associated with soil indigenous microbiota and fertilization regimes

Abstract

Keywords

Access to Document

Fingerprint

Cite this