Efficient cryopreservation of human pluripotent stem cells by surface-based vitrification

TY - JOUR

T1 - Efficient cryopreservation of human pluripotent stem cells by surface-based vitrification

AU - Neubauer, Julia C

AU - Beier, Axel F

AU - Geijsen, Niels

AU - Zimmermann, Heiko

PY - 2015

Y1 - 2015

N2 - Efficient cryopreservation of human stem cells is crucial for guaranteeing a permanent supply of high-quality cell material for drug discovery or regenerative medicine. Conventionally used protocols usually employing slow freezing rates, however, result in low recovery rates for human pluripotent stem cells due to their complex colony structure. In this chapter, a surface-based vitrification protocol for pluripotent stem cells is presented based on a procedure for human embryonic stem cells developed by Beier et al. (Cryobiology 63:175-185, 2011). This simple and highly efficient cryopreservation method allows cryopreservation of large numbers of ready-to-use adherent cells that maintain pluripotency.

AB - Efficient cryopreservation of human stem cells is crucial for guaranteeing a permanent supply of high-quality cell material for drug discovery or regenerative medicine. Conventionally used protocols usually employing slow freezing rates, however, result in low recovery rates for human pluripotent stem cells due to their complex colony structure. In this chapter, a surface-based vitrification protocol for pluripotent stem cells is presented based on a procedure for human embryonic stem cells developed by Beier et al. (Cryobiology 63:175-185, 2011). This simple and highly efficient cryopreservation method allows cryopreservation of large numbers of ready-to-use adherent cells that maintain pluripotency.

U2 - 10.1007/978-1-4939-2193-5_13

DO - 10.1007/978-1-4939-2193-5_13

M3 - Article

C2 - 25428013

SN - 1064-3745

VL - 1257

SP - 321

EP - 328

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -