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Elevated Immune Gene Expression Is Associated with Poor Reproductive Success of Urban Blue Tits. / Capilla-Lasheras, Pablo (Corresponding author); Dominoni, D.M.; Babayan, Simon; O'Shaughnessy, Peter; Mladenova, Magdalena ; Woodford, Luke; Pollock, Christopher J.; Barr, Tom; Baldini, Francesco; Helm, Barbara.

In: Frontiers in Ecology and Evolution, 16.06.2017.

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Harvard

Capilla-Lasheras, P, Dominoni, DM, Babayan, S, O'Shaughnessy, P, Mladenova, M, Woodford, L, Pollock, CJ, Barr, T, Baldini, F & Helm, B 2017, 'Elevated Immune Gene Expression Is Associated with Poor Reproductive Success of Urban Blue Tits' Frontiers in Ecology and Evolution. https://doi.org/10.3389/fevo.2017.00064

APA

Capilla-Lasheras, P., Dominoni, D. M., Babayan, S., O'Shaughnessy, P., Mladenova, M., Woodford, L., ... Helm, B. (2017). Elevated Immune Gene Expression Is Associated with Poor Reproductive Success of Urban Blue Tits. Frontiers in Ecology and Evolution. https://doi.org/10.3389/fevo.2017.00064

Vancouver

Capilla-Lasheras P, Dominoni DM, Babayan S, O'Shaughnessy P, Mladenova M, Woodford L et al. Elevated Immune Gene Expression Is Associated with Poor Reproductive Success of Urban Blue Tits. Frontiers in Ecology and Evolution. 2017 Jun 16. https://doi.org/10.3389/fevo.2017.00064

Author

Capilla-Lasheras, Pablo ; Dominoni, D.M. ; Babayan, Simon ; O'Shaughnessy, Peter ; Mladenova, Magdalena ; Woodford, Luke ; Pollock, Christopher J. ; Barr, Tom ; Baldini, Francesco ; Helm, Barbara. / Elevated Immune Gene Expression Is Associated with Poor Reproductive Success of Urban Blue Tits. In: Frontiers in Ecology and Evolution. 2017.

BibTeX

@article{5ecfe20104df49538aed63855faef00d,
title = "Elevated Immune Gene Expression Is Associated with Poor Reproductive Success of Urban Blue Tits",
abstract = "Urban and forest habitats differ in many aspects that can lead to modifications of the immune system of wild animals. Altered parasite communities, pollution, and artificial light at night in cities have been associated with exacerbated inflammatory responses, with possibly negative fitness consequences, but few data are available from free-living animals. Here, we investigate how urbanization affects major immune pathways and experimentally test potentially contributing factors in blue tits (Cyanistes caeruleus) from an urban and forest site. We first compared breeding adults by quantifying the mRNA transcript levels of proteins associated with anti-bacterial, anti-malarial (TLR4, LY86) and anti-helminthic (Type 2 transcription factor GATA3) immune responses. Adult urban and forest blue tits differed in gene expression, with significantly increased TLR4 and GATA3, but not LY86, in the city. We then experimentally tested whether these differences were environmentally induced by cross-fostering eggs between the sites and measuring mRNA transcripts in nestlings. The populations differed in reduced reproductive success, with a lower fledging success and lower fledgling weight recorded at the urban site. This mirrors the findings of our twin study reporting that the urban site was severely resource limited when compared to the forest. Because of low urban survival, robust gene expression data were only obtained from nestlings reared in the forest. Transcript levels in these nestlings showed no (TLR4, LY86), or weak (GATA3), differences according to their origin from forest or city nests, suggesting little genetic or maternal contribution to nestling immune transcript levels. Lastly, to investigate differences in parasite pressure between urban and forest sites, we measured the prevalence of malaria in adult and nestling blood. Prevalence was invariably high across environments and not associated with the transcript levels of the studied immune genes. Our results support the hypothesis that inflammatory pathways are activated in an urban environment and suggest that these differences are most likely induced by environmental factors.",
keywords = "international",
author = "Pablo Capilla-Lasheras and D.M. Dominoni and Simon Babayan and Peter O'Shaughnessy and Magdalena Mladenova and Luke Woodford and Pollock, {Christopher J.} and Tom Barr and Francesco Baldini and Barbara Helm",
note = "6385, AnE; Data Archiving: no data data archived at University of Glasgow",
year = "2017",
month = "6",
day = "16",
doi = "10.3389/fevo.2017.00064",
language = "English",
journal = "Frontiers in Ecology and Evolution",
issn = "2296-701X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Elevated Immune Gene Expression Is Associated with Poor Reproductive Success of Urban Blue Tits

AU - Capilla-Lasheras, Pablo

AU - Dominoni, D.M.

AU - Babayan, Simon

AU - O'Shaughnessy, Peter

AU - Mladenova, Magdalena

AU - Woodford, Luke

AU - Pollock, Christopher J.

AU - Barr, Tom

AU - Baldini, Francesco

AU - Helm, Barbara

N1 - 6385, AnE; Data Archiving: no data data archived at University of Glasgow

PY - 2017/6/16

Y1 - 2017/6/16

N2 - Urban and forest habitats differ in many aspects that can lead to modifications of the immune system of wild animals. Altered parasite communities, pollution, and artificial light at night in cities have been associated with exacerbated inflammatory responses, with possibly negative fitness consequences, but few data are available from free-living animals. Here, we investigate how urbanization affects major immune pathways and experimentally test potentially contributing factors in blue tits (Cyanistes caeruleus) from an urban and forest site. We first compared breeding adults by quantifying the mRNA transcript levels of proteins associated with anti-bacterial, anti-malarial (TLR4, LY86) and anti-helminthic (Type 2 transcription factor GATA3) immune responses. Adult urban and forest blue tits differed in gene expression, with significantly increased TLR4 and GATA3, but not LY86, in the city. We then experimentally tested whether these differences were environmentally induced by cross-fostering eggs between the sites and measuring mRNA transcripts in nestlings. The populations differed in reduced reproductive success, with a lower fledging success and lower fledgling weight recorded at the urban site. This mirrors the findings of our twin study reporting that the urban site was severely resource limited when compared to the forest. Because of low urban survival, robust gene expression data were only obtained from nestlings reared in the forest. Transcript levels in these nestlings showed no (TLR4, LY86), or weak (GATA3), differences according to their origin from forest or city nests, suggesting little genetic or maternal contribution to nestling immune transcript levels. Lastly, to investigate differences in parasite pressure between urban and forest sites, we measured the prevalence of malaria in adult and nestling blood. Prevalence was invariably high across environments and not associated with the transcript levels of the studied immune genes. Our results support the hypothesis that inflammatory pathways are activated in an urban environment and suggest that these differences are most likely induced by environmental factors.

AB - Urban and forest habitats differ in many aspects that can lead to modifications of the immune system of wild animals. Altered parasite communities, pollution, and artificial light at night in cities have been associated with exacerbated inflammatory responses, with possibly negative fitness consequences, but few data are available from free-living animals. Here, we investigate how urbanization affects major immune pathways and experimentally test potentially contributing factors in blue tits (Cyanistes caeruleus) from an urban and forest site. We first compared breeding adults by quantifying the mRNA transcript levels of proteins associated with anti-bacterial, anti-malarial (TLR4, LY86) and anti-helminthic (Type 2 transcription factor GATA3) immune responses. Adult urban and forest blue tits differed in gene expression, with significantly increased TLR4 and GATA3, but not LY86, in the city. We then experimentally tested whether these differences were environmentally induced by cross-fostering eggs between the sites and measuring mRNA transcripts in nestlings. The populations differed in reduced reproductive success, with a lower fledging success and lower fledgling weight recorded at the urban site. This mirrors the findings of our twin study reporting that the urban site was severely resource limited when compared to the forest. Because of low urban survival, robust gene expression data were only obtained from nestlings reared in the forest. Transcript levels in these nestlings showed no (TLR4, LY86), or weak (GATA3), differences according to their origin from forest or city nests, suggesting little genetic or maternal contribution to nestling immune transcript levels. Lastly, to investigate differences in parasite pressure between urban and forest sites, we measured the prevalence of malaria in adult and nestling blood. Prevalence was invariably high across environments and not associated with the transcript levels of the studied immune genes. Our results support the hypothesis that inflammatory pathways are activated in an urban environment and suggest that these differences are most likely induced by environmental factors.

KW - international

U2 - 10.3389/fevo.2017.00064

DO - 10.3389/fevo.2017.00064

M3 - Article

JO - Frontiers in Ecology and Evolution

JF - Frontiers in Ecology and Evolution

SN - 2296-701X

ER -

ID: 5528071