Enhancement of production of pathogen suppressing volatiles by precursor molecules: towards sustainable control of soilborne diseases.

Christina Papazlatani; Annabell Wagner; W. de Boer; Paolina Garbeva

Enhancement of production of pathogen suppressing volatiles by precursor molecules: towards sustainable control of soilborne diseases.

Christina Papazlatani, Annabell Wagner, W. de Boer, Paolina Garbeva

NIOO - Microbial Ecology (ME)

Research output: Contribution to conference › Poster › Scientific

Abstract

Bacterial volatile organic compounds (VOCs) can play a significant role in antagonistic interactions between microorganisms. Enhancing the production of bacterial VOCs suppressing the growth of soil-borne plant pathogenic fungi, has perspective as a sustainable disease control strategy. In the present study, we explored the potential of stimulating production of pathogen suppressing VOCs by provision of precursor molecules, such as amino acids. Burkholderia AD24 was supplied with different amino acids, in mixture and individually, and the composition and suppressive effect of the volatile blend against Fusarium culmorum and Rhizoctonia solani was evaluated. Coincident presence of different amino acids in the bacterial growth medium, resulted in higher suppression of both pathogens via produced volatiles. Subsequent analysis of the composition of the volatile blend produced by Burkholderia AD24 in the presence and absence of amino acids, showed higher abundance of antifungal compounds in the former, including sulfur compounds (DMDS) and heterocyclic oxygen compounds (dioxane, dioxolane). Follow-up treatment with single amino acids revealed a pathogen specific effect, where F. culmorum was significantly suppressed by the volatile blend produced when Burkholderia AD24 was cultivated in the presence of glutamine, asparagine and arginine, whereas R. solani was suppressed in the presence of glycine. Analysis of the volatile blend composition also showed differences between the different amino acid treatments. Our results show that precursor molecules, like amino acids, can enhance the production of pathogen suppressing bacterial VOCs. Next step, will be to test this under conditions used in practice.

Original language	English
Publication status	Published - 01 Dec 2023

Cite this

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title = "Enhancement of production of pathogen suppressing volatiles by precursor molecules: towards sustainable control of soilborne diseases.",

abstract = "Bacterial volatile organic compounds (VOCs) can play a significant role in antagonistic interactions between microorganisms. Enhancing the production of bacterial VOCs suppressing the growth of soil-borne plant pathogenic fungi, has perspective as a sustainable disease control strategy. In the present study, we explored the potential of stimulating production of pathogen suppressing VOCs by provision of precursor molecules, such as amino acids. Burkholderia AD24 was supplied with different amino acids, in mixture and individually, and the composition and suppressive effect of the volatile blend against Fusarium culmorum and Rhizoctonia solani was evaluated. Coincident presence of different amino acids in the bacterial growth medium, resulted in higher suppression of both pathogens via produced volatiles. Subsequent analysis of the composition of the volatile blend produced by Burkholderia AD24 in the presence and absence of amino acids, showed higher abundance of antifungal compounds in the former, including sulfur compounds (DMDS) and heterocyclic oxygen compounds (dioxane, dioxolane). Follow-up treatment with single amino acids revealed a pathogen specific effect, where F. culmorum was significantly suppressed by the volatile blend produced when Burkholderia AD24 was cultivated in the presence of glutamine, asparagine and arginine, whereas R. solani was suppressed in the presence of glycine. Analysis of the volatile blend composition also showed differences between the different amino acid treatments. Our results show that precursor molecules, like amino acids, can enhance the production of pathogen suppressing bacterial VOCs. Next step, will be to test this under conditions used in practice.",

author = "Christina Papazlatani and Annabell Wagner and {de Boer}, W. and Paolina Garbeva",

year = "2023",

month = dec,

day = "1",

language = "English",

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TY - CONF

T1 - Enhancement of production of pathogen suppressing volatiles by precursor molecules: towards sustainable control of soilborne diseases.

AU - Papazlatani, Christina

AU - Wagner, Annabell

AU - de Boer, W.

AU - Garbeva, Paolina

PY - 2023/12/1

Y1 - 2023/12/1

N2 - Bacterial volatile organic compounds (VOCs) can play a significant role in antagonistic interactions between microorganisms. Enhancing the production of bacterial VOCs suppressing the growth of soil-borne plant pathogenic fungi, has perspective as a sustainable disease control strategy. In the present study, we explored the potential of stimulating production of pathogen suppressing VOCs by provision of precursor molecules, such as amino acids. Burkholderia AD24 was supplied with different amino acids, in mixture and individually, and the composition and suppressive effect of the volatile blend against Fusarium culmorum and Rhizoctonia solani was evaluated. Coincident presence of different amino acids in the bacterial growth medium, resulted in higher suppression of both pathogens via produced volatiles. Subsequent analysis of the composition of the volatile blend produced by Burkholderia AD24 in the presence and absence of amino acids, showed higher abundance of antifungal compounds in the former, including sulfur compounds (DMDS) and heterocyclic oxygen compounds (dioxane, dioxolane). Follow-up treatment with single amino acids revealed a pathogen specific effect, where F. culmorum was significantly suppressed by the volatile blend produced when Burkholderia AD24 was cultivated in the presence of glutamine, asparagine and arginine, whereas R. solani was suppressed in the presence of glycine. Analysis of the volatile blend composition also showed differences between the different amino acid treatments. Our results show that precursor molecules, like amino acids, can enhance the production of pathogen suppressing bacterial VOCs. Next step, will be to test this under conditions used in practice.

AB - Bacterial volatile organic compounds (VOCs) can play a significant role in antagonistic interactions between microorganisms. Enhancing the production of bacterial VOCs suppressing the growth of soil-borne plant pathogenic fungi, has perspective as a sustainable disease control strategy. In the present study, we explored the potential of stimulating production of pathogen suppressing VOCs by provision of precursor molecules, such as amino acids. Burkholderia AD24 was supplied with different amino acids, in mixture and individually, and the composition and suppressive effect of the volatile blend against Fusarium culmorum and Rhizoctonia solani was evaluated. Coincident presence of different amino acids in the bacterial growth medium, resulted in higher suppression of both pathogens via produced volatiles. Subsequent analysis of the composition of the volatile blend produced by Burkholderia AD24 in the presence and absence of amino acids, showed higher abundance of antifungal compounds in the former, including sulfur compounds (DMDS) and heterocyclic oxygen compounds (dioxane, dioxolane). Follow-up treatment with single amino acids revealed a pathogen specific effect, where F. culmorum was significantly suppressed by the volatile blend produced when Burkholderia AD24 was cultivated in the presence of glutamine, asparagine and arginine, whereas R. solani was suppressed in the presence of glycine. Analysis of the volatile blend composition also showed differences between the different amino acid treatments. Our results show that precursor molecules, like amino acids, can enhance the production of pathogen suppressing bacterial VOCs. Next step, will be to test this under conditions used in practice.

M3 - Poster

ER -