Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger

Yanping Jiang; Alexandra Vivas Duarte; Joost van den Brink; Ad Wiebenga; Gen Zou; Chengshu Wang; Ronald P de Vries; Zhihua Zhou; Isabelle Benoit

doi:10.1007/s10529-015-1951-9

Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger

Yanping Jiang, Alexandra Vivas Duarte, Joost van den Brink, Ad Wiebenga, Gen Zou, Chengshu Wang, Ronald P de Vries, Zhihua Zhou, Isabelle Benoit

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

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Abstract

OBJECTIVES: To increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals.

RESULTS: By overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-D-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3 IU/ml, respectively. The sugar yield of wheat straw saccharification by combining enzymes from this mutant and the Aspergillus niger genetically modified strain ΔcreA/xlnR c/araR c was improved up to 7.5 mg/ml, a 229 % increase compared to the combination of wild type strains.

CONCLUSIONS: Mixing enzymes from T. reesei and A. niger combined with the genetic modification of transcription factors is a promising strategy to increase saccharification efficiency.

Original language	English
Pages (from-to)	65-70
Number of pages	6
Journal	Biotechnology Letters
Volume	38
Issue number	1
DOIs	https://doi.org/10.1007/s10529-015-1951-9
Publication status	Published - Jan 2016

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title = "Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger",

abstract = "OBJECTIVES: To increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals.RESULTS: By overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-D-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3 IU/ml, respectively. The sugar yield of wheat straw saccharification by combining enzymes from this mutant and the Aspergillus niger genetically modified strain ΔcreA/xlnR c/araR c was improved up to 7.5 mg/ml, a 229 % increase compared to the combination of wild type strains.CONCLUSIONS: Mixing enzymes from T. reesei and A. niger combined with the genetic modification of transcription factors is a promising strategy to increase saccharification efficiency.",

author = "Yanping Jiang and Duarte, {Alexandra Vivas} and {van den Brink}, Joost and Ad Wiebenga and Gen Zou and Chengshu Wang and {de Vries}, {Ronald P} and Zhihua Zhou and Isabelle Benoit",

year = "2016",

month = jan,

doi = "10.1007/s10529-015-1951-9",

language = "English",

volume = "38",

pages = "65--70",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "1",

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TY - JOUR

T1 - Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger

AU - Jiang, Yanping

AU - Duarte, Alexandra Vivas

AU - van den Brink, Joost

AU - Wiebenga, Ad

AU - Zou, Gen

AU - Wang, Chengshu

AU - de Vries, Ronald P

AU - Zhou, Zhihua

AU - Benoit, Isabelle

PY - 2016/1

Y1 - 2016/1

N2 - OBJECTIVES: To increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals.RESULTS: By overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-D-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3 IU/ml, respectively. The sugar yield of wheat straw saccharification by combining enzymes from this mutant and the Aspergillus niger genetically modified strain ΔcreA/xlnR c/araR c was improved up to 7.5 mg/ml, a 229 % increase compared to the combination of wild type strains.CONCLUSIONS: Mixing enzymes from T. reesei and A. niger combined with the genetic modification of transcription factors is a promising strategy to increase saccharification efficiency.

AB - OBJECTIVES: To increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals.RESULTS: By overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-D-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3 IU/ml, respectively. The sugar yield of wheat straw saccharification by combining enzymes from this mutant and the Aspergillus niger genetically modified strain ΔcreA/xlnR c/araR c was improved up to 7.5 mg/ml, a 229 % increase compared to the combination of wild type strains.CONCLUSIONS: Mixing enzymes from T. reesei and A. niger combined with the genetic modification of transcription factors is a promising strategy to increase saccharification efficiency.

U2 - 10.1007/s10529-015-1951-9

DO - 10.1007/s10529-015-1951-9

M3 - Article

C2 - 26354856

SN - 0141-5492

VL - 38

SP - 65

EP - 70

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 1

ER -

Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger

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