Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and Aspergillus niger

Yanping Jiang, Alexandra Vivas Duarte, Joost van den Brink, Ad Wiebenga, Gen Zou, Chengshu Wang, Ronald P de Vries, Zhihua Zhou, Isabelle Benoit

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    OBJECTIVES: To increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals.

    RESULTS: By overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-D-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3 IU/ml, respectively. The sugar yield of wheat straw saccharification by combining enzymes from this mutant and the Aspergillus niger genetically modified strain ΔcreA/xlnR c/araR c was improved up to 7.5 mg/ml, a 229 % increase compared to the combination of wild type strains.

    CONCLUSIONS: Mixing enzymes from T. reesei and A. niger combined with the genetic modification of transcription factors is a promising strategy to increase saccharification efficiency.

    Original languageEnglish
    Pages (from-to)65-70
    Number of pages6
    JournalBiotechnology Letters
    Volume38
    Issue number1
    DOIs
    Publication statusPublished - Jan 2016

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