ENU mutagenesis to generate genetically modified rat models

R. van Boxtel, M. Gould, E. Cuppen, B.M. Smits

Research output: Contribution to journal/periodicalArticleScientificpeer-review

26 Citations (Scopus)


The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.
Original languageEnglish
Pages (from-to)151-167
JournalMethods in Molecular Biology
Publication statusPublished - 2010


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