• 6038_vanGurp

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We describe epiGBS, a reduced representation bisulfite sequencing method for cost-effective exploration and comparative analysis of DNA methylation and genetic variation in hundreds of samples de novo. This method uses genotyping by sequencing of bisulfite-converted DNA followed by reliable de novo reference construction, mapping, variant calling, and distinction of single-nucleotide polymorphisms (SNPs) versus methylation variation (software is available at The output can be loaded directly into a genome browser for visualization and into RnBeads for analysis of differential methylation.

Original languageEnglish
Pages (from-to)322-324
JournalNature Methods
Issue number4
Publication statusPublished - 2016

    Research areas

  • national

ID: 1798825