Establishment of compatibility in the Ustilago maydis/maize pathosystem

Gunther Doehlemann, Ramon Wahl, Miroslav Vranes, Ronald P de Vries, Jörg Kämper, Regine Kahmann

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    The fungus Ustilago maydis is a biotrophic pathogen parasitizing on maize. The most prominent symptoms of the disease are large tumors in which fungal proliferation and spore differentiation occur. In this study, we have analyzed early and late tumor stages by confocal microscopy. We show that fungal differentiation occurs both within plant cells as well as in cavities where huge aggregates of fungal mycelium develop. U. maydis is poorly equipped with plant CWDEs and we demonstrate by array analysis that the respective genes follow distinct expression profiles at early and late stages of tumor development. For the set of three genes coding for pectinolytic enzymes, deletion mutants were generated by gene replacement. Neither single nor triple mutants were affected in pathogenic development. Based on our studies, we consider it unlikely that U. maydis feeds on carbohydrates derived from the digestion of plant cell wall material, but uses its set of plant CWDEs for softening the cell wall structure as a prerequisite for in planta growth.

    Original languageEnglish
    Pages (from-to)29-40
    Number of pages12
    JournalJournal of Plant Physiology
    Volume165
    Issue number1
    DOIs
    Publication statusPublished - Jan 2008

    Keywords

    • Gene Expression Profiling
    • Gene Expression Regulation, Plant
    • Genes, Plant
    • Host-Pathogen Interactions
    • Microscopy, Confocal
    • Pectins
    • Plant Diseases
    • Plant Leaves
    • Ustilago
    • Zea mays

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