Evaluating Mechanisms of Soil Microbiome Suppression of Striga Infection in Sorghum

Tamera Taylor; Jiregna Daksa; Mahdere Z. Shimels; Desalegn W. Etalo; Benjamin Thiombiano; Aimee Walmsey; Alexander J. Chen; Harro J. Bouwmeester; Jos M. Raaijmakers; Siobhan M. Brady; Dorota Kawa

doi:10.21769/BioProtoc.5058

Evaluating Mechanisms of Soil Microbiome Suppression of Striga Infection in Sorghum

Tamera Taylor, Jiregna Daksa, Mahdere Z. Shimels, Desalegn W. Etalo, Benjamin Thiombiano, Aimee Walmsey, Alexander J. Chen, Harro J. Bouwmeester, Jos M. Raaijmakers, Siobhan M. Brady, Dorota Kawa^* (Corresponding author)

^*Corresponding author for this work

NIOO - Microbial Ecology (ME)

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

The root parasitic weed Striga hermonthica has a devastating effect on sorghum and other cereal crops in Sub-Saharan Africa. Available Striga management strategies are rarely sufficient or not widely accessible or affordable. Identification of soil- or plant-associated microorganisms that interfere in the Striga infection cycle holds potential for development of complementary biological control measures. Such inoculants should be preferably based on microbes native to the regions of their application. We developed a method to assess microbiome-based soil suppressiveness to Striga with a minimal amount of field-collected soil. We previously used this method to identify the mechanisms of microbe-mediated suppression of Striga infection and to test individual microbial strains. Here, we present protocols to assess the functional potential of the soil microbiome and individual bacterial taxa that adversely affect Striga parasitism in sorghum via three major known suppression mechanisms. These methods can be further extended to other Striga hosts and other root parasitic weeds.

Original language	English
Article number	e5058
Journal	Bio-Protocol
Volume	14
Issue number	17
DOIs	https://doi.org/10.21769/BioProtoc.5058
Publication status	Published - 05 Sept 2024

Keywords

Aerenchyma
Haustorium-inducing factors
Microbiome
Sorghum
Striga hermonthica
Suberin
Suppressive soils

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10.21769/BioProtoc.5058Licence: CC BY

Cite this

@article{88348493452b4617a4aeb45f587dd794,

title = "Evaluating Mechanisms of Soil Microbiome Suppression of Striga Infection in Sorghum",

abstract = "The root parasitic weed Striga hermonthica has a devastating effect on sorghum and other cereal crops in Sub-Saharan Africa. Available Striga management strategies are rarely sufficient or not widely accessible or affordable. Identification of soil- or plant-associated microorganisms that interfere in the Striga infection cycle holds potential for development of complementary biological control measures. Such inoculants should be preferably based on microbes native to the regions of their application. We developed a method to assess microbiome-based soil suppressiveness to Striga with a minimal amount of field-collected soil. We previously used this method to identify the mechanisms of microbe-mediated suppression of Striga infection and to test individual microbial strains. Here, we present protocols to assess the functional potential of the soil microbiome and individual bacterial taxa that adversely affect Striga parasitism in sorghum via three major known suppression mechanisms. These methods can be further extended to other Striga hosts and other root parasitic weeds.",

keywords = "Aerenchyma, Haustorium-inducing factors, Microbiome, Sorghum, Striga hermonthica, Suberin, Suppressive soils",

author = "Tamera Taylor and Jiregna Daksa and Shimels, {Mahdere Z.} and Etalo, {Desalegn W.} and Benjamin Thiombiano and Aimee Walmsey and Chen, {Alexander J.} and Bouwmeester, {Harro J.} and Raaijmakers, {Jos M.} and Brady, {Siobhan M.} and Dorota Kawa",

note = "Data archiving: no data",

year = "2024",

month = sep,

day = "5",

doi = "10.21769/BioProtoc.5058",

language = "English",

volume = "14",

journal = "Bio-Protocol",

issn = "2331-8325",

publisher = "Bio-Protocol",

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TY - JOUR

T1 - Evaluating Mechanisms of Soil Microbiome Suppression of Striga Infection in Sorghum

AU - Taylor, Tamera

AU - Daksa, Jiregna

AU - Shimels, Mahdere Z.

AU - Etalo, Desalegn W.

AU - Thiombiano, Benjamin

AU - Walmsey, Aimee

AU - Chen, Alexander J.

AU - Bouwmeester, Harro J.

AU - Raaijmakers, Jos M.

AU - Brady, Siobhan M.

AU - Kawa, Dorota

N1 - Data archiving: no data

PY - 2024/9/5

Y1 - 2024/9/5

N2 - The root parasitic weed Striga hermonthica has a devastating effect on sorghum and other cereal crops in Sub-Saharan Africa. Available Striga management strategies are rarely sufficient or not widely accessible or affordable. Identification of soil- or plant-associated microorganisms that interfere in the Striga infection cycle holds potential for development of complementary biological control measures. Such inoculants should be preferably based on microbes native to the regions of their application. We developed a method to assess microbiome-based soil suppressiveness to Striga with a minimal amount of field-collected soil. We previously used this method to identify the mechanisms of microbe-mediated suppression of Striga infection and to test individual microbial strains. Here, we present protocols to assess the functional potential of the soil microbiome and individual bacterial taxa that adversely affect Striga parasitism in sorghum via three major known suppression mechanisms. These methods can be further extended to other Striga hosts and other root parasitic weeds.

AB - The root parasitic weed Striga hermonthica has a devastating effect on sorghum and other cereal crops in Sub-Saharan Africa. Available Striga management strategies are rarely sufficient or not widely accessible or affordable. Identification of soil- or plant-associated microorganisms that interfere in the Striga infection cycle holds potential for development of complementary biological control measures. Such inoculants should be preferably based on microbes native to the regions of their application. We developed a method to assess microbiome-based soil suppressiveness to Striga with a minimal amount of field-collected soil. We previously used this method to identify the mechanisms of microbe-mediated suppression of Striga infection and to test individual microbial strains. Here, we present protocols to assess the functional potential of the soil microbiome and individual bacterial taxa that adversely affect Striga parasitism in sorghum via three major known suppression mechanisms. These methods can be further extended to other Striga hosts and other root parasitic weeds.

KW - Aerenchyma

KW - Haustorium-inducing factors

KW - Microbiome

KW - Sorghum

KW - Striga hermonthica

KW - Suberin

KW - Suppressive soils

U2 - 10.21769/BioProtoc.5058

DO - 10.21769/BioProtoc.5058

M3 - Article

AN - SCOPUS:85203444605

SN - 2331-8325

VL - 14

JO - Bio-Protocol

JF - Bio-Protocol

IS - 17

M1 - e5058

ER -