Experimental evidence for the impact of phages on mineralization of soil-derived dissolved organic matter under different temperature regimes

TY - JOUR

T1 - Experimental evidence for the impact of phages on mineralization of soil-derived dissolved organic matter under different temperature regimes

AU - Wang, Shuang

AU - Yu, Senxiang

AU - Zhao, Xiaoyan

AU - Zhao, Xiaolei

AU - Mason-Jones, Kyle

AU - Zhu, Zhenke

AU - Redmile-Gordon, Marc

AU - Li, Yong

AU - Chen, Jianping

AU - Kuzyakov, Yakov

AU - Ge, Tida

N1 - Publisher Copyright: © 2022 Elsevier B.V.

PY - 2022

Y1 - 2022

N2 - Microbial mineralization of dissolved organic matter (DOM) plays an important role in regulating C and nutrient cycling. Viruses are the most abundant biological agents on Earth, but their effect on the density and activity of soil microorganisms and, consequently, on mineralization of DOM under different temperatures remains poorly understood. To assess the impact of viruses on DOM mineralization, we added soil phage concentrate (active vs. inactive phage control) to four DOM extracts containing inoculated microbial communities and incubated them at 18 °C and 23 °C for 32 days. Infection with active phages generally decreased DOM mineralization at day one and showed accelerated DOM mineralization later (especially from day 5 to 15) compared to that with the inactivated phages. Overall, phage infection increased the microbially driven CO2 release. Notably, while higher temperature increased the total CO2 release, the cumulative CO2 release induced by phage infection (difference between active phages and inactivated control) was not affected. However, higher temperatures advanced the response time of the phages but shortening its active period. Our findings suggest that bacterial predation by phages can significantly affect soil DOM mineralization. Therefore, higher temperatures may accelerate host-phage interactions and thus, the duration of C recycling.

AB - Microbial mineralization of dissolved organic matter (DOM) plays an important role in regulating C and nutrient cycling. Viruses are the most abundant biological agents on Earth, but their effect on the density and activity of soil microorganisms and, consequently, on mineralization of DOM under different temperatures remains poorly understood. To assess the impact of viruses on DOM mineralization, we added soil phage concentrate (active vs. inactive phage control) to four DOM extracts containing inoculated microbial communities and incubated them at 18 °C and 23 °C for 32 days. Infection with active phages generally decreased DOM mineralization at day one and showed accelerated DOM mineralization later (especially from day 5 to 15) compared to that with the inactivated phages. Overall, phage infection increased the microbially driven CO2 release. Notably, while higher temperature increased the total CO2 release, the cumulative CO2 release induced by phage infection (difference between active phages and inactivated control) was not affected. However, higher temperatures advanced the response time of the phages but shortening its active period. Our findings suggest that bacterial predation by phages can significantly affect soil DOM mineralization. Therefore, higher temperatures may accelerate host-phage interactions and thus, the duration of C recycling.

KW - Carbon cycle

KW - Dissolved organic carbon

KW - Soil phages

KW - Viral shunt

KW - Warming

U2 - 10.1016/j.scitotenv.2022.157517

DO - 10.1016/j.scitotenv.2022.157517

M3 - Article

C2 - 35872205

AN - SCOPUS:85134794481

SN - 0048-9697

VL - 846

JO - Science of the Total Environment

JF - Science of the Total Environment

M1 - 157517

ER -