Exploitation of new endophytic bacteria and their ability to promote sugarcane growth and nitrogen nutrition

TY - JOUR

T1 - Exploitation of new endophytic bacteria and their ability to promote sugarcane growth and nitrogen nutrition

AU - da Silveira, A.P.D.

AU - Iório, Raquel de Paula Freitas Iório

AU - Marcos, F.C.C.

AU - Fernandes, A.O.

AU - De Souza, S.A.C.D.

AU - Kuramae, E.E.

AU - Cipriano, Matheus

N1 - 6596, ME; Data Archiving: no NIOO data

PY - 2019

Y1 - 2019

N2 - Few studies have evaluated endophytic bacteria in relation to plant growth promotion, nitrogen uptake and biological control. The aim of this study was to molecularly and physiologically characterize thirteen endophytic bacteria strains, evaluate their biological control properties and their ability to promote plant growth and plant N nutrition. All the strains produced indole acetic acid and promoted increase of plant biomass, N accumulative amount and N-use efficiency index. None of the strains carries the nifH gene. Four strains stimulated plant nitrate reductase activity, four solubilized phosphate, nine produced siderophores and none produced HCN. Seven strains inhibited Bipolaris sacchari growth and one was antagonistic to Ceratocystis paradoxa. The pathogens were inhibited by the production of diffusible and volatile metabolites by the bacterial strains. Moreover, this is the first study to demonstrate the effect of Delftia acidovorans on sugarcane plant growth, nitrogen metabolism improvement and antagonism to B. sacchari. The most efficient strains in promoting plant growth and exhibiting antagonistic activities towards fungal pathogens were Herbaspirillum frinsingense (IAC-BECa-152) and three Pantoea dispersa strains (IAC-BECa-128, IAC-BECa-129, and IAC-BECa-132). These bacteria show potential to be used as inoculants for sustainable agricultural management, mainly at the seedling production phase.

AB - Few studies have evaluated endophytic bacteria in relation to plant growth promotion, nitrogen uptake and biological control. The aim of this study was to molecularly and physiologically characterize thirteen endophytic bacteria strains, evaluate their biological control properties and their ability to promote plant growth and plant N nutrition. All the strains produced indole acetic acid and promoted increase of plant biomass, N accumulative amount and N-use efficiency index. None of the strains carries the nifH gene. Four strains stimulated plant nitrate reductase activity, four solubilized phosphate, nine produced siderophores and none produced HCN. Seven strains inhibited Bipolaris sacchari growth and one was antagonistic to Ceratocystis paradoxa. The pathogens were inhibited by the production of diffusible and volatile metabolites by the bacterial strains. Moreover, this is the first study to demonstrate the effect of Delftia acidovorans on sugarcane plant growth, nitrogen metabolism improvement and antagonism to B. sacchari. The most efficient strains in promoting plant growth and exhibiting antagonistic activities towards fungal pathogens were Herbaspirillum frinsingense (IAC-BECa-152) and three Pantoea dispersa strains (IAC-BECa-128, IAC-BECa-129, and IAC-BECa-132). These bacteria show potential to be used as inoculants for sustainable agricultural management, mainly at the seedling production phase.

KW - international

KW - Plant growth-promoting bacteria

KW - Nitrate reductase

KW - Saccharum sp

KW - Indole acetic acid

KW - Delftia acidovorans

KW - Fungal pathogen antagonism

U2 - 10.1007/s10482-018-1157-y

DO - 10.1007/s10482-018-1157-y

M3 - Article

SN - 0003-6072

VL - 112

SP - 283

EP - 295

JO - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology

JF - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology

IS - 2

ER -