Exploring the Volatiles Released from Roots of Wild and Domesticated Tomato Plants under Insect Attack

TY - JOUR

T1 - Exploring the Volatiles Released from Roots of Wild and Domesticated Tomato Plants under Insect Attack

AU - Díaz, Ana Shein Lee

AU - Rizaludin, Muhammad Syamsu

AU - Zweers, Hans

AU - Raaijmakers, Jos M.

AU - Garbeva, Paolina

N1 - 7402, ME; Data archiving: data in Zenodo

PY - 2022

Y1 - 2022

N2 - Plants produce volatile organic compounds that are important in communication and defense. While studies have largely focused on volatiles emitted from aboveground plant parts upon exposure to biotic or abiotic stresses, volatile emissions from roots upon aboveground stress are less studied. Here, we investigated if tomato plants under insect herbivore attack exhibited a different root volatilome than non-stressed plants, and whether this was influenced by the plant’s genetic background. To this end, we analyzed one domesticated and one wild tomato species, i.e., Solanum lycopersicum cv Moneymaker and Solanum pimpinellifolium, respectively, exposed to leaf herbivory by the insect Spodoptera exigua. Root volatiles were trapped with two sorbent materials, HiSorb and PDMS, at 24 h after exposure to insect stress. Our results revealed that differences in root volatilome were species-, stress-, and material-dependent. Upon leaf herbivory, the domesticated and wild tomato species showed different root volatile profiles. The wild species presented the largest change in root volatile compounds with an overall reduction in monoterpene emission under stress. Similarly, the domesticated species presented a slight reduction in monoterpene emission and an increased production of fatty-acid-derived volatiles under stress. Volatile profiles differed between the two sorbent materials, and both were required to obtain a more comprehensive characterization of the root volatilome. Collectively, these results provide a strong basis to further unravel the impact of herbivory stress on systemic volatile emissions.

AB - Plants produce volatile organic compounds that are important in communication and defense. While studies have largely focused on volatiles emitted from aboveground plant parts upon exposure to biotic or abiotic stresses, volatile emissions from roots upon aboveground stress are less studied. Here, we investigated if tomato plants under insect herbivore attack exhibited a different root volatilome than non-stressed plants, and whether this was influenced by the plant’s genetic background. To this end, we analyzed one domesticated and one wild tomato species, i.e., Solanum lycopersicum cv Moneymaker and Solanum pimpinellifolium, respectively, exposed to leaf herbivory by the insect Spodoptera exigua. Root volatiles were trapped with two sorbent materials, HiSorb and PDMS, at 24 h after exposure to insect stress. Our results revealed that differences in root volatilome were species-, stress-, and material-dependent. Upon leaf herbivory, the domesticated and wild tomato species showed different root volatile profiles. The wild species presented the largest change in root volatile compounds with an overall reduction in monoterpene emission under stress. Similarly, the domesticated species presented a slight reduction in monoterpene emission and an increased production of fatty-acid-derived volatiles under stress. Volatile profiles differed between the two sorbent materials, and both were required to obtain a more comprehensive characterization of the root volatilome. Collectively, these results provide a strong basis to further unravel the impact of herbivory stress on systemic volatile emissions.

KW - Benzyl alcohol

KW - HiSorb

KW - Induced defenses

KW - Insect herbivory

KW - Methyl salicylate

KW - Monoterpene

KW - PDMS

KW - Root volatiles

KW - Solanum lycopersicum

KW - Solanum pimpinellifolium

KW - Tomato domestication

KW - NIOO

KW - Plan_S-Compliant-OA

UR - https://doi.org/10.5281/zenodo.5788930

U2 - 10.3390/molecules27051612

DO - 10.3390/molecules27051612

M3 - Article

C2 - 35268714

AN - SCOPUS:85126028846

SN - 1420-3049

VL - 27

JO - Molecules

JF - Molecules

IS - 5

M1 - 1612

ER -