Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

Giovanni Giuseppe Giobbe; Claire Crowley; Camilla Luni; Sara Campinoti; Moustafa Khedr; Kai Kretzschmar; Martina Maria De Santis; Elisa Zambaiti; Federica Michielin; Laween Meran; Qianjiang Hu; Gijs van Son; Luca Urbani; Anna Manfredi; Monica Giomo; Simon Eaton; Davide Cacchiarelli; Vivian S W Li; Hans Clevers; Paola Bonfanti; Nicola Elvassore; Paolo De Coppi

doi:10.1038/s41467-019-13605-4

Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

Giovanni Giuseppe Giobbe, Claire Crowley, Camilla Luni, Sara Campinoti, Moustafa Khedr, Kai Kretzschmar, Martina Maria De Santis, Elisa Zambaiti, Federica Michielin, Laween Meran, Qianjiang Hu, Gijs van Son, Luca Urbani, Anna Manfredi, Monica Giomo, Simon Eaton, Davide Cacchiarelli, Vivian S W Li, Hans Clevers, Paola BonfantiNicola Elvassore, Paolo De Coppi

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

317 Citations (Scopus)

Abstract

Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

Original language	English
Pages (from-to)	5658
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-13605-4
Publication status	Published - 11 Dec 2019

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Giobbe, G. G., Crowley, C., Luni, C., Campinoti, S., Khedr, M., Kretzschmar, K., De Santis, M. M., Zambaiti, E., Michielin, F., Meran, L., Hu, Q., van Son, G., Urbani, L., Manfredi, A., Giomo, M., Eaton, S., Cacchiarelli, D., Li, V. S. W., Clevers, H., ... De Coppi, P. (2019). Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture. Nature Communications, 10(1), 5658. https://doi.org/10.1038/s41467-019-13605-4

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title = "Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture",

abstract = "Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.",

author = "Giobbe, {Giovanni Giuseppe} and Claire Crowley and Camilla Luni and Sara Campinoti and Moustafa Khedr and Kai Kretzschmar and {De Santis}, {Martina Maria} and Elisa Zambaiti and Federica Michielin and Laween Meran and Qianjiang Hu and {van Son}, Gijs and Luca Urbani and Anna Manfredi and Monica Giomo and Simon Eaton and Davide Cacchiarelli and Li, {Vivian S W} and Hans Clevers and Paola Bonfanti and Nicola Elvassore and {De Coppi}, Paolo",

year = "2019",

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day = "11",

doi = "10.1038/s41467-019-13605-4",

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Giobbe, GG, Crowley, C, Luni, C, Campinoti, S, Khedr, M, Kretzschmar, K, De Santis, MM, Zambaiti, E, Michielin, F, Meran, L, Hu, Q, van Son, G, Urbani, L, Manfredi, A, Giomo, M, Eaton, S, Cacchiarelli, D, Li, VSW, Clevers, H, Bonfanti, P, Elvassore, N & De Coppi, P 2019, 'Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture', Nature Communications, vol. 10, no. 1, pp. 5658. https://doi.org/10.1038/s41467-019-13605-4

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T1 - Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

AU - Giobbe, Giovanni Giuseppe

AU - Crowley, Claire

AU - Luni, Camilla

AU - Campinoti, Sara

AU - Khedr, Moustafa

AU - Kretzschmar, Kai

AU - De Santis, Martina Maria

AU - Zambaiti, Elisa

AU - Michielin, Federica

AU - Meran, Laween

AU - Hu, Qianjiang

AU - van Son, Gijs

AU - Urbani, Luca

AU - Manfredi, Anna

AU - Giomo, Monica

AU - Eaton, Simon

AU - Cacchiarelli, Davide

AU - Li, Vivian S W

AU - Clevers, Hans

AU - Bonfanti, Paola

AU - Elvassore, Nicola

AU - De Coppi, Paolo

PY - 2019/12/11

Y1 - 2019/12/11

N2 - Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

AB - Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

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DO - 10.1038/s41467-019-13605-4

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VL - 10

SP - 5658

JO - Nature Communications

JF - Nature Communications

IS - 1

ER -

Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

Abstract

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