FRET-based Stoichiometry Measurements of Protein Complexes

Francesca Mattiroli, Yajie Gu, Karolin Luger

Research output: Contribution to journal/periodicalArticleScientificpeer-review


For a complete understanding of biochemical reactions, information on complex stoichiometry is essential. However, measuring stoichiometry is experimentally challenging. Our lab has developed a FRET-based assay to study protein complex stoichiometry in vitro. This assay, also known as Job plot, is set up as a continuous variation of the molar ratio between the two species, kept at constant total concentration. The FRET (Fluorescence Resonance Energy Transfer) between the two fluorescently-labeled proteins is measured and the stoichiometry is inferred from the sample with highest FRET signal. This approach allows us to assess complex stoichiometry in solution.

Original languageEnglish
Issue number3
Publication statusPublished - 05 Feb 2018


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