FRET-based Stoichiometry Measurements of Protein Complexes

Francesca Mattiroli, Yajie Gu, Karolin Luger

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

For a complete understanding of biochemical reactions, information on complex stoichiometry is essential. However, measuring stoichiometry is experimentally challenging. Our lab has developed a FRET-based assay to study protein complex stoichiometry in vitro. This assay, also known as Job plot, is set up as a continuous variation of the molar ratio between the two species, kept at constant total concentration. The FRET (Fluorescence Resonance Energy Transfer) between the two fluorescently-labeled proteins is measured and the stoichiometry is inferred from the sample with highest FRET signal. This approach allows us to assess complex stoichiometry in solution.

Original languageEnglish
JournalBio-Protocol
Volume7
Issue number3
DOIs
Publication statusPublished - 05 Feb 2018

Fingerprint

Dive into the research topics of 'FRET-based Stoichiometry Measurements of Protein Complexes'. Together they form a unique fingerprint.

Cite this