Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization

Adiphol Dilokpimol, Miia R Mäkelä, Gabriella Cerullo, Miaomiao Zhou, Simona Varriale, Loknath Gidijala, Joana L A Brás, Peter Jütten, Alexander Piechot, Raymond Verhaert, Vincenza Faraco, Kristiina S. Hilden, Ronald P de Vries

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

4-O-Methyl-d-glucuronic acid (MeGlcA) is a side-residue of glucuronoarabinoxylan and can form ester linkages to lignin, contributing significantly to the strength and rigidity of the plant cell wall. Glucuronoyl esterases (4-O-methyl-glucuronoyl methylesterases, GEs) can cleave this ester bond, and therefore may play a significant role as auxiliary enzymes in biomass saccharification for the production of biofuels and biochemicals. GEs belong to a relatively new family of carbohydrate esterases (CE15) in the CAZy database (www.cazy.org), and so far around ten fungal GEs have been characterized. To explore additional GE enzymes, we used a genome mining strategy. BLAST analysis with characterized GEs against approximately 250 publicly accessible fungal genomes identified more than 150 putative fungal GEs, which were classified into eight phylogenetic sub-groups. To validate the genome mining strategy, 21 selected GEs from both ascomycete and basidiomycete fungi were heterologously produced in Pichia pastoris. Of these enzymes, 18 were active against benzyl d-glucuronate demonstrating the suitability of our genome mining strategy for enzyme discovery.

Original languageEnglish
Pages (from-to)282-287
Number of pages6
JournalNew Biotechnology
Volume40
Issue numberPt B
DOIs
Publication statusPublished - 25 Jan 2018

Keywords

  • Journal Article

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    Dilokpimol, A., Mäkelä, M. R., Cerullo, G., Zhou, M., Varriale, S., Gidijala, L., Brás, J. L. A., Jütten, P., Piechot, A., Verhaert, R., Faraco, V., Hilden, K. S., & de Vries, R. P. (2018). Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization. New Biotechnology, 40(Pt B), 282-287. https://doi.org/10.1016/j.nbt.2017.10.003