Fungal volatile compounds induce production of the secondary metabolite Sodorifen in Serratia plymuthica PRI-2C

R.L. Schmidt; V.C.L. de Jager; D. Zühlke; C. Wolff; J. Bernhardt; Katarina Cankar; J. Beekwilder; W. van Ijcken; Frank Sleutels; W. De Boer; K. Riedel; P.V. Garbeva

doi:10.1038/s41598-017-00893-3

Fungal volatile compounds induce production of the secondary metabolite Sodorifen in Serratia plymuthica PRI-2C

R.L. Schmidt (Corresponding author), V.C.L. de Jager, D. Zühlke, C. Wolff, J. Bernhardt, Katarina Cankar, J. Beekwilder, W. van Ijcken, Frank Sleutels, W. De Boer, K. Riedel, P.V. Garbeva (Corresponding author)

NIOO - Microbial Ecology (ME)

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Abstract

The ability of bacteria and fungi to communicate with each other is a remarkable aspect of the microbial world. It is recognized that volatile organic compounds (VOCs) act as communication signals, however the molecular responses by bacteria to fungal VOCs remain unknown. Here we perform transcriptomics and proteomics analyses of Serratia plymuthica PRI-2C exposed to VOCs emitted by the fungal pathogen Fusarium culmorum. We find that the bacterium responds to fungal VOCs with changes in gene and protein expression related to motility, signal transduction, energy metabolism, cell envelope biogenesis, and secondary metabolite production. Metabolomic analysis of the bacterium exposed to the fungal VOCs, gene cluster comparison, and heterologous co-expression of a terpene synthase and a methyltransferase revealed the production of the unusual terpene sodorifen in response to fungal VOCs. These results strongly suggest that VOCs are not only a metabolic waste but important compounds in the long-distance communication between fungi and bacteria

Original language	English
Article number	862
Journal	Scientific Reports
Volume	7
Early online date	13 Apr 2017
DOIs	https://doi.org/10.1038/s41598-017-00893-3
Publication status	Published - 2017

Keywords

international

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6238_Schmidt_AMAccepted author manuscript, 12.2 MBLicence: CC BY
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Schmidt, R. L., de Jager, V. C. L., Zühlke, D., Wolff, C., Bernhardt, J., Cankar, K., Beekwilder, J., van Ijcken, W., Sleutels, F., De Boer, W., Riedel, K., & Garbeva, P. V. (2017). Fungal volatile compounds induce production of the secondary metabolite Sodorifen in Serratia plymuthica PRI-2C. Scientific Reports, 7, Article 862 . https://doi.org/10.1038/s41598-017-00893-3

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title = "Fungal volatile compounds induce production of the secondary metabolite Sodorifen in Serratia plymuthica PRI-2C",

abstract = "The ability of bacteria and fungi to communicate with each other is a remarkable aspect of the microbial world. It is recognized that volatile organic compounds (VOCs) act as communication signals, however the molecular responses by bacteria to fungal VOCs remain unknown. Here we perform transcriptomics and proteomics analyses of Serratia plymuthica PRI-2C exposed to VOCs emitted by the fungal pathogen Fusarium culmorum. We find that the bacterium responds to fungal VOCs with changes in gene and protein expression related to motility, signal transduction, energy metabolism, cell envelope biogenesis, and secondary metabolite production. Metabolomic analysis of the bacterium exposed to the fungal VOCs, gene cluster comparison, and heterologous co-expression of a terpene synthase and a methyltransferase revealed the production of the unusual terpene sodorifen in response to fungal VOCs. These results strongly suggest that VOCs are not only a metabolic waste but important compounds in the long-distance communication between fungi and bacteria",

keywords = "international",

author = "R.L. Schmidt and {de Jager}, V.C.L. and D. Z{\"u}hlke and C. Wolff and J. Bernhardt and Katarina Cankar and J. Beekwilder and {van Ijcken}, W. and Frank Sleutels and {De Boer}, W. and K. Riedel and P.V. Garbeva",

note = "6238, ME; Data Archiving: no data: The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository.",

year = "2017",

doi = "10.1038/s41598-017-00893-3",

language = "English",

volume = "7",

journal = "Scientific Reports",

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T1 - Fungal volatile compounds induce production of the secondary metabolite Sodorifen in Serratia plymuthica PRI-2C

AU - Schmidt, R.L.

AU - de Jager, V.C.L.

AU - Zühlke, D.

AU - Wolff, C.

AU - Bernhardt, J.

AU - Cankar, Katarina

AU - Beekwilder, J.

AU - van Ijcken, W.

AU - Sleutels, Frank

AU - De Boer, W.

AU - Riedel, K.

AU - Garbeva, P.V.

N1 - 6238, ME; Data Archiving: no data: The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository.

PY - 2017

Y1 - 2017

N2 - The ability of bacteria and fungi to communicate with each other is a remarkable aspect of the microbial world. It is recognized that volatile organic compounds (VOCs) act as communication signals, however the molecular responses by bacteria to fungal VOCs remain unknown. Here we perform transcriptomics and proteomics analyses of Serratia plymuthica PRI-2C exposed to VOCs emitted by the fungal pathogen Fusarium culmorum. We find that the bacterium responds to fungal VOCs with changes in gene and protein expression related to motility, signal transduction, energy metabolism, cell envelope biogenesis, and secondary metabolite production. Metabolomic analysis of the bacterium exposed to the fungal VOCs, gene cluster comparison, and heterologous co-expression of a terpene synthase and a methyltransferase revealed the production of the unusual terpene sodorifen in response to fungal VOCs. These results strongly suggest that VOCs are not only a metabolic waste but important compounds in the long-distance communication between fungi and bacteria

AB - The ability of bacteria and fungi to communicate with each other is a remarkable aspect of the microbial world. It is recognized that volatile organic compounds (VOCs) act as communication signals, however the molecular responses by bacteria to fungal VOCs remain unknown. Here we perform transcriptomics and proteomics analyses of Serratia plymuthica PRI-2C exposed to VOCs emitted by the fungal pathogen Fusarium culmorum. We find that the bacterium responds to fungal VOCs with changes in gene and protein expression related to motility, signal transduction, energy metabolism, cell envelope biogenesis, and secondary metabolite production. Metabolomic analysis of the bacterium exposed to the fungal VOCs, gene cluster comparison, and heterologous co-expression of a terpene synthase and a methyltransferase revealed the production of the unusual terpene sodorifen in response to fungal VOCs. These results strongly suggest that VOCs are not only a metabolic waste but important compounds in the long-distance communication between fungi and bacteria

KW - international

U2 - 10.1038/s41598-017-00893-3

DO - 10.1038/s41598-017-00893-3

M3 - Article

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 862

ER -

Fungal volatile compounds induce production of the secondary metabolite Sodorifen in Serratia plymuthica PRI-2C

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