GAPDH as a control gene to estimate genome copy number in Great Tits, with cross-amplification in Blue Tits

TY - JOUR

T1 - GAPDH as a control gene to estimate genome copy number in Great Tits, with cross-amplification in Blue Tits

AU - Atema, E.

AU - Van Oers, K.

AU - Verhulst, S.

N1 - Reporting year: 2013 Metis note: 5438;AnE; Data archiving: data archived at MDA

PY - 2013

Y1 - 2013

N2 - Estimating the number of genome copies in a tissue sample can serve various purposes. For example, such an estimate serves as scaling variable when measuring telomeres with quantitative PCR. We describe the primer development and evaluation for the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene in the Great Tit Parus major, as a control gene to estimate genome copy number. We demonstrate specific amplification with negligible variation in 48 Great Tits and cross-amplification in 53 Blue Tits Cyanistes caeruleus. We conclude this primer set to be reliable for amplification of GAPDH as a reference gene for quantitative PCR analysis in Great and Blue Tits.

AB - Estimating the number of genome copies in a tissue sample can serve various purposes. For example, such an estimate serves as scaling variable when measuring telomeres with quantitative PCR. We describe the primer development and evaluation for the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene in the Great Tit Parus major, as a control gene to estimate genome copy number. We demonstrate specific amplification with negligible variation in 48 Great Tits and cross-amplification in 53 Blue Tits Cyanistes caeruleus. We conclude this primer set to be reliable for amplification of GAPDH as a reference gene for quantitative PCR analysis in Great and Blue Tits.

KW - national

U2 - 10.5253/078.101.0107

DO - 10.5253/078.101.0107

M3 - Article

SN - 0373-2266

VL - 101

SP - 49

EP - 54

JO - Ardea

JF - Ardea

IS - 1

ER -