Generation of BAC Transgenic Epithelial Organoids

G. Schwank, A. Andersson-Rolf, B.K. Koo, N. Sasaki, H. Clevers

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Under previously developed culture conditions, mouse and human intestinal epithelia can be cultured and expanded over long periods. These so-called organoids recapitulate the three-dimensional architecture of the gut epithelium, and consist of all major intestinal cell types. One key advantage of these ex vivo cultures is their accessibility to live imaging. So far the establishment of transgenic fluorescent reporter organoids has required the generation of transgenic mice, a laborious and time-consuming process, which cannot be extended to human cultures. Here we present a transfection protocol that enables the generation of recombinant mouse and human reporter organoids using BAC (bacterial artificial chromosome) technology.
Original languageEnglish
Pages (from-to)76871
JournalPLoS One
Volume8
Issue number10
DOIs
Publication statusPublished - 2013

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