Genetic diversity and pathogenicity of Botryosphaeriaceae species associated with symptomatic Citrus plants in Europe

Jadson Diogo Pereira Bezerra, Pedro Wilhelm Crous, Dalia Aiello, Maria Lodovica Gullino, Giancarlo Polizzi, Vladimiro Guarnaccia

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

This study represents the first survey studying the occurrence, genetic diversity, and pathogenicity of Botryosphaeriaceae species associated with symptomatic citrus species in citrus-production areas in five European countries. Based on morphological features and phylogenetic analyses of internal transcribed spacer (ITS) of nuclear ribosomal DNA (nrDNA), translation elongation factor 1-alpha (TEF1) and β-tubulin (TUB2) genes, nine species were identified as belonging to the genera Diplodia, Dothiorella, Lasiodiplodia, and Neofusicoccum. Isolates of Neofusicoccum parvum and Diplodia pseudoseriata were the most frequently detected, while Dothiorella viticola had the widest distribution, occurring in four of the five countries sampled. Representative isolates of the nine Botryosphaeriaceae species used in the pathogenicity tests caused similar symptoms to those observed in nature. Isolates assayed were all re-isolated, thereby fulfilling Koch’s postulates. Isolates of Diplodia pseudoseriata and Diplodia olivarum are recorded for the first time on citrus and all species found in our study, except N. parvum, are reported for the first time on citrus in Europe.
Original languageEnglish
Article number492
Number of pages31
JournalPlants
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 2021

Keywords

  • Diplodia
  • Dothiorella
  • Lasiodiplodia
  • Neofusicoccum
  • pathogenic fungi
  • phylogeny

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