Genetic, phenotypic and metabolic diversity of yeasts from wheat flag leaves

TY - JOUR

T1 - Genetic, phenotypic and metabolic diversity of yeasts from wheat flag leaves

AU - Gouka, Linda

AU - Vogels, Caroline

AU - Hansen, Lars H.

AU - Raaijmakers, J.M.

AU - Cordovez, Viviane

N1 - 7454, ME; Data archiving: ENA, PRJEB51687

PY - 2022

Y1 - 2022

N2 - The phyllosphere, the aboveground part of a plant, is a harsh environment with diverse abiotic and biotic stresses, including oscillating nutrient availability and temperature as well as exposure to UV radiation. Microbial colonization of this dynamic environment requires specific adaptive traits, including tolerance to fluctuating temperatures, the production of secondary metabolites and pigments to successfully compete with other microorganisms and to withstand abiotic stresses. Here, we isolated 175 yeasts, comprising 15 different genera, from the wheat flag leaf and characterized a selection of these for various adaptive traits such as substrate utilization, tolerance to different temperatures, biofilm formation, and antagonism towards the fungal leaf pathogen Fusarium graminearum. Collectively our results revealed that the wheat flag leaf is a rich resource of taxonomically and phenotypically diverse yeast genera that exhibit various traits that can contribute to survival in the harsh phyllosphere environment.

AB - The phyllosphere, the aboveground part of a plant, is a harsh environment with diverse abiotic and biotic stresses, including oscillating nutrient availability and temperature as well as exposure to UV radiation. Microbial colonization of this dynamic environment requires specific adaptive traits, including tolerance to fluctuating temperatures, the production of secondary metabolites and pigments to successfully compete with other microorganisms and to withstand abiotic stresses. Here, we isolated 175 yeasts, comprising 15 different genera, from the wheat flag leaf and characterized a selection of these for various adaptive traits such as substrate utilization, tolerance to different temperatures, biofilm formation, and antagonism towards the fungal leaf pathogen Fusarium graminearum. Collectively our results revealed that the wheat flag leaf is a rich resource of taxonomically and phenotypically diverse yeast genera that exhibit various traits that can contribute to survival in the harsh phyllosphere environment.

UR - https://www.ebi.ac.uk/ena/browser/view/PRJEB51687

U2 - 10.3389/fpls.2022.908628

DO - 10.3389/fpls.2022.908628

M3 - Article

SN - 1664-462X

VL - 13

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

M1 - 908628

ER -