Genome Analysis of a Newly Discovered Yeast Species, Hanseniaspora menglaensis

Adam P. Ryan*, Marizeth Groenewald, Maudy Th Smith, Cian Holohan, Teun Boekhout, Kenneth H. Wolfe, Geraldine Butler

*Corresponding author for this work

Research output: Contribution to journal/periodicalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Annual surveys of Irish soil samples identified three isolates, CBS 16921 (UCD88), CBS 18246 (UCD443), and CBS 18247 (UCD483), of an apiculate yeast species within the Hanseniaspora genus. The internal transcribed spacer (ITS) and D1/D2 region of the large subunit (LSU) rRNA sequences showed that these are isolates of the recently described species Hanseniaspora menglaensis, first isolated from Southwest China. No genome sequence for H. menglaensis is currently available. The genome sequences of the three Irish isolates were determined using short-read (Illumina) sequencing, and the sequence of one isolate (CBS 16921) was assembled to chromosome level using long-read sequencing (Oxford Nanopore Technologies). Phylogenomic analysis shows that H. menglaensis belongs to the fast-evolving lineage (FEL) of Hanseniaspora. Only one MAT idiomorph (encoding MATα1) was identified in all three sequenced H. menglaensis isolates, consistent with one mating type of a heterothallic species. Genome comparisons showed that there has been a rearrangement near MATα of FEL species compared to isolates from the slowly evolving lineage (SEL).

Original languageEnglish
Article number180
JournalJournal of Fungi
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 2024

Keywords

  • chromosomes
  • fast-evolving lineage
  • genome assembly
  • heterothallic
  • Illumina sequencing
  • MAT locus
  • nanopore sequencing
  • phylogenomics
  • sporulation
  • yeast mitochondrion

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