Genome editing by natural and engineered CRISPR-associated nucleases

Wen Y Wu; Joyce H G Lebbink; Roland Kanaar; Niels Geijsen; John van der Oost

doi:10.1038/s41589-018-0080-x

Genome editing by natural and engineered CRISPR-associated nucleases

Wen Y Wu, Joyce H G Lebbink, Roland Kanaar, Niels Geijsen, John van der Oost

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of single-polypeptide CRISPR-associated systems have been discovered including Cas12a/Cpf1 and Cas13a/C2c2. Despite distant similarities to Cas9, these additional systems have unique structural and functional features, providing new opportunities for genome editing applications. Here, relevant fundamental features of natural and engineered CRISPR-Cas variants are compared. Moreover, practical matters are discussed that are essential for dedicated genome editing applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity.

Original language	English
Pages (from-to)	642-651
Number of pages	10
Journal	Nature Chemical Biology
Volume	14
Issue number	7
DOIs	https://doi.org/10.1038/s41589-018-0080-x
Publication status	Published - Jul 2018

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abstract = "Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of single-polypeptide CRISPR-associated systems have been discovered including Cas12a/Cpf1 and Cas13a/C2c2. Despite distant similarities to Cas9, these additional systems have unique structural and functional features, providing new opportunities for genome editing applications. Here, relevant fundamental features of natural and engineered CRISPR-Cas variants are compared. Moreover, practical matters are discussed that are essential for dedicated genome editing applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity.",

author = "Wu, {Wen Y} and Lebbink, {Joyce H G} and Roland Kanaar and Niels Geijsen and {van der Oost}, John",

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AB - Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of single-polypeptide CRISPR-associated systems have been discovered including Cas12a/Cpf1 and Cas13a/C2c2. Despite distant similarities to Cas9, these additional systems have unique structural and functional features, providing new opportunities for genome editing applications. Here, relevant fundamental features of natural and engineered CRISPR-Cas variants are compared. Moreover, practical matters are discussed that are essential for dedicated genome editing applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity.

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Genome editing by natural and engineered CRISPR-associated nucleases

Abstract

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