Genome editing by natural and engineered CRISPR-associated nucleases

Wen Y Wu, Joyce H G Lebbink, Roland Kanaar, Niels Geijsen, John van der Oost

Research output: Contribution to journal/periodicalArticleScientificpeer-review


Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of single-polypeptide CRISPR-associated systems have been discovered including Cas12a/Cpf1 and Cas13a/C2c2. Despite distant similarities to Cas9, these additional systems have unique structural and functional features, providing new opportunities for genome editing applications. Here, relevant fundamental features of natural and engineered CRISPR-Cas variants are compared. Moreover, practical matters are discussed that are essential for dedicated genome editing applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity.

Original languageEnglish
Pages (from-to)642-651
Number of pages10
JournalNature Chemical Biology
Issue number7
Publication statusPublished - Jul 2018


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