Genome-wide identification of 31 cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and analysis of their benzo[alpha]pyrene-induced expression patterns

Jeonghoon Han; Duck-Hyun Kim; Hui-Su Kim; Hee-Jin Kim; S.A.J. Declerck; Atsushi Hagiwara; Jae-Seong Lee

doi:10.1016/j.cbd.2017.10.003

Genome-wide identification of 31 cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and analysis of their benzo[alpha]pyrene-induced expression patterns

Jeonghoon Han, Duck-Hyun Kim, Hui-Su Kim, Hee-Jin Kim, S.A.J. Declerck, Atsushi Hagiwara, Jae-Seong Lee (Corresponding author)

NIOO - Aquatic Ecology (AqE)

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Abstract

While marine invertebrate cytochrome P450 (CYP) genes and their roles in detoxification mechanisms have been studied, little information is available regarding freshwater rotifer CYPs and their functions. Here, we used genomic sequences and RNA-seq databases to identify 31 CYP genes in the freshwater rotifer Brachionus calyciflorus. The 31 Bc-CYP genes with a few tandem duplications were clustered into CYP 2, 3, 4, mitochondrial, and 46 clans with two marine rotifers Brachionus plicatilis and Brachionus koreanus. To understand the molecular responses of these 31 Bc-CYP genes, we also examined their expression patterns in response to benzo[α]pyrene (B[α]P). Three Bc-CYP genes (Bc-CYP3044B3, Bc-CYP3049B4, Bc-CYP3049B6) were significantly upregulated (P < 0.05) in response to B[α]P, suggesting that these CYP genes can be involved in detoxification in response to B[α]P exposure. These genes might be useful as biomarkers of B[α]P exposure in B. calyciflorus. Overall, our findings expand the repertoire of known CYPs and shed light on their potential roles in xenobiotic detoxification in rotifers.

Original language	English
Pages (from-to)	26-33
Journal	Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume	25
Issue number	March
DOIs	https://doi.org/10.1016/j.cbd.2017.10.003
Publication status	Published - 2018

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international

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Han, J., Kim, D.-H., Kim, H.-S., Kim, H.-J., Declerck, S. A. J., Hagiwara, A., & Lee, J.-S. (2018). Genome-wide identification of 31 cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and analysis of their benzo[alpha]pyrene-induced expression patterns. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 25(March), 26-33. https://doi.org/10.1016/j.cbd.2017.10.003

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title = "Genome-wide identification of 31 cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and analysis of their benzo[alpha]pyrene-induced expression patterns",

abstract = "While marine invertebrate cytochrome P450 (CYP) genes and their roles in detoxification mechanisms have been studied, little information is available regarding freshwater rotifer CYPs and their functions. Here, we used genomic sequences and RNA-seq databases to identify 31 CYP genes in the freshwater rotifer Brachionus calyciflorus. The 31 Bc-CYP genes with a few tandem duplications were clustered into CYP 2, 3, 4, mitochondrial, and 46 clans with two marine rotifers Brachionus plicatilis and Brachionus koreanus. To understand the molecular responses of these 31 Bc-CYP genes, we also examined their expression patterns in response to benzo[α]pyrene (B[α]P). Three Bc-CYP genes (Bc-CYP3044B3, Bc-CYP3049B4, Bc-CYP3049B6) were significantly upregulated (P < 0.05) in response to B[α]P, suggesting that these CYP genes can be involved in detoxification in response to B[α]P exposure. These genes might be useful as biomarkers of B[α]P exposure in B. calyciflorus. Overall, our findings expand the repertoire of known CYPs and shed light on their potential roles in xenobiotic detoxification in rotifers.",

keywords = "international ",

author = "Jeonghoon Han and Duck-Hyun Kim and Hui-Su Kim and Hee-Jin Kim and S.A.J. Declerck and Atsushi Hagiwara and Jae-Seong Lee",

note = "6292, AqE; Data archiving: no NIOO data",

year = "2018",

doi = "10.1016/j.cbd.2017.10.003",

language = "English",

volume = "25",

pages = "26--33",

journal = "Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics",

issn = "1744-117X",

publisher = "Elsevier B.V.",

number = "March",

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Han, J, Kim, D-H, Kim, H-S, Kim, H-J, Declerck, SAJ, Hagiwara, A & Lee, J-S 2018, 'Genome-wide identification of 31 cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and analysis of their benzo[alpha]pyrene-induced expression patterns', Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, vol. 25, no. March, pp. 26-33. https://doi.org/10.1016/j.cbd.2017.10.003

Genome-wide identification of 31 cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and analysis of their benzo[alpha]pyrene-induced expression patterns. / Han, Jeonghoon; Kim, Duck-Hyun; Kim, Hui-Su et al.
In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 25, No. March, 2018, p. 26-33.

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

TY - JOUR

T1 - Genome-wide identification of 31 cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and analysis of their benzo[alpha]pyrene-induced expression patterns

AU - Han, Jeonghoon

AU - Kim, Duck-Hyun

AU - Kim, Hui-Su

AU - Kim, Hee-Jin

AU - Declerck, S.A.J.

AU - Hagiwara, Atsushi

AU - Lee, Jae-Seong

N1 - 6292, AqE; Data archiving: no NIOO data

PY - 2018

Y1 - 2018

N2 - While marine invertebrate cytochrome P450 (CYP) genes and their roles in detoxification mechanisms have been studied, little information is available regarding freshwater rotifer CYPs and their functions. Here, we used genomic sequences and RNA-seq databases to identify 31 CYP genes in the freshwater rotifer Brachionus calyciflorus. The 31 Bc-CYP genes with a few tandem duplications were clustered into CYP 2, 3, 4, mitochondrial, and 46 clans with two marine rotifers Brachionus plicatilis and Brachionus koreanus. To understand the molecular responses of these 31 Bc-CYP genes, we also examined their expression patterns in response to benzo[α]pyrene (B[α]P). Three Bc-CYP genes (Bc-CYP3044B3, Bc-CYP3049B4, Bc-CYP3049B6) were significantly upregulated (P < 0.05) in response to B[α]P, suggesting that these CYP genes can be involved in detoxification in response to B[α]P exposure. These genes might be useful as biomarkers of B[α]P exposure in B. calyciflorus. Overall, our findings expand the repertoire of known CYPs and shed light on their potential roles in xenobiotic detoxification in rotifers.

AB - While marine invertebrate cytochrome P450 (CYP) genes and their roles in detoxification mechanisms have been studied, little information is available regarding freshwater rotifer CYPs and their functions. Here, we used genomic sequences and RNA-seq databases to identify 31 CYP genes in the freshwater rotifer Brachionus calyciflorus. The 31 Bc-CYP genes with a few tandem duplications were clustered into CYP 2, 3, 4, mitochondrial, and 46 clans with two marine rotifers Brachionus plicatilis and Brachionus koreanus. To understand the molecular responses of these 31 Bc-CYP genes, we also examined their expression patterns in response to benzo[α]pyrene (B[α]P). Three Bc-CYP genes (Bc-CYP3044B3, Bc-CYP3049B4, Bc-CYP3049B6) were significantly upregulated (P < 0.05) in response to B[α]P, suggesting that these CYP genes can be involved in detoxification in response to B[α]P exposure. These genes might be useful as biomarkers of B[α]P exposure in B. calyciflorus. Overall, our findings expand the repertoire of known CYPs and shed light on their potential roles in xenobiotic detoxification in rotifers.

KW - international

U2 - 10.1016/j.cbd.2017.10.003

DO - 10.1016/j.cbd.2017.10.003

M3 - Article

SN - 1744-117X

VL - 25

SP - 26

EP - 33

JO - Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics

JF - Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics

IS - March

ER -

Han J, Kim DH, Kim HS, Kim HJ, Declerck SAJ, Hagiwara A et al. Genome-wide identification of 31 cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and analysis of their benzo[alpha]pyrene-induced expression patterns. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 2018;25(March):26-33. doi: 10.1016/j.cbd.2017.10.003

Genome-wide identification of 31 cytochrome P450 (CYP) genes in the freshwater rotifer Brachionus calyciflorus and analysis of their benzo[alpha]pyrene-induced expression patterns

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