Heterogeneity in kinesin function

Babu J N Reddy, Suvranta Tripathy, Michael Vershinin, Marvin E Tanenbaum, Jing Xu, Michelle Mattson-Hoss, Karim Arabi, Dail Chapman, Tory Doolin, Changbong Hyeon, Steven P Gross

Research output: Contribution to journal/periodicalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

The kinesin family proteins are often studied as prototypical molecular motors; a deeper understanding of them can illuminate regulation of intracellular transport. It is typically assumed that they function identically. Here we find that this assumption of homogeneous function appears incorrect: variation among motors' velocities in vivo and in vitro is larger than the stochastic variation expected for an ensemble of "identical" motors. When moving on microtubules, slow and fast motors are persistently slow, and fast, respectively. We develop theory that provides quantitative criteria to determine whether the observed single-molecule variation is too large to be generated from an ensemble of identical molecules. To analyze such heterogeneity, we group traces into homogeneous sub-ensembles. Motility studies varying the temperature, pH and glycerol concentration suggest at least 2 distinct functional states that are independently affected by external conditions. We end by investigating the functional ramifications of such heterogeneity through Monte-Carlo multi-motor simulations.

Original languageEnglish
Pages (from-to)658-671
Number of pages14
JournalTraffic
Volume18
Issue number10
DOIs
Publication statusPublished - Oct 2017

Keywords

  • Journal Article

Fingerprint

Dive into the research topics of 'Heterogeneity in kinesin function'. Together they form a unique fingerprint.

Cite this