High potential low-cost crude enzymes from Algerian fungal strains for plant polysaccharides hydrolysis

Ourdia Bouzid; Amina Allouache; Ronald P. de Vries; Dalila Zitouni; Isabelle Benoit-Gelber; Jos Houbraken; Majda Amina Aziza

doi:10.1080/17597269.2023.2295661

High potential low-cost crude enzymes from Algerian fungal strains for plant polysaccharides hydrolysis

Ourdia Bouzid^*, Amina Allouache, Ronald P. de Vries, Dalila Zitouni, Isabelle Benoit-Gelber, Jos Houbraken, Majda Amina Aziza

^*Corresponding author for this work

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

Fungi secrete large enzyme sets, such as glycosyl hydrolases (GH) capable of targeting plant polymers, making them a valuable enzymes source. In this study, three fungal strains were isolated from the rhizosphere area and identified as Trichoderma harzianum, Aspergillus tubingensis and Aspergillus tamarii by molecular analysis. The strains were able to grow on mono-, di- and polysaccharides as sole carbon sources, and even on D-galactose and cellulose which is rare for Aspergillus species. Besides, GH activities such as cellobiohydrolase, β-1,4-D-galactosidase, β-mannosidase glucoamylase and β-1,4-D-galactosidase were revealed in secretome of the strains cultivated on wheat bran with optimum obtained between 4th and 7th day. Furthermore, hydrolysis of wheat bran with T. harzianum, A. tubingensis and A. tamarii culture supernatant resulted in the release of 4.6, 5.5 and6.8 g/L of glucose, respectively. The yield increased two-fold by applying a mix of T. harzianum and A. tubingensis enzymes extracts at a ratio 1:1. In addition, the combination of the crude enzymes with commercial cellulase enhanced 3 times the glucose content. The results present enzyme extracts with high yield in saccharification of complex substrates with a potential application in bioethanol production.

Original language	English
Pages (from-to)	767-772
Number of pages	6
Journal	Biofuels
Volume	15
Issue number	7
DOIs	https://doi.org/10.1080/17597269.2023.2295661
Publication status	Published - 2024

Keywords

cellulases
Fungi
hemicellulases
italique
saccharification
Trichoderma
wheat bran

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10.1080/17597269.2023.2295661

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title = "High potential low-cost crude enzymes from Algerian fungal strains for plant polysaccharides hydrolysis",

abstract = "Fungi secrete large enzyme sets, such as glycosyl hydrolases (GH) capable of targeting plant polymers, making them a valuable enzymes source. In this study, three fungal strains were isolated from the rhizosphere area and identified as Trichoderma harzianum, Aspergillus tubingensis and Aspergillus tamarii by molecular analysis. The strains were able to grow on mono-, di- and polysaccharides as sole carbon sources, and even on D-galactose and cellulose which is rare for Aspergillus species. Besides, GH activities such as cellobiohydrolase, β-1,4-D-galactosidase, β-mannosidase glucoamylase and β-1,4-D-galactosidase were revealed in secretome of the strains cultivated on wheat bran with optimum obtained between 4th and 7th day. Furthermore, hydrolysis of wheat bran with T. harzianum, A. tubingensis and A. tamarii culture supernatant resulted in the release of 4.6, 5.5 and6.8 g/L of glucose, respectively. The yield increased two-fold by applying a mix of T. harzianum and A. tubingensis enzymes extracts at a ratio 1:1. In addition, the combination of the crude enzymes with commercial cellulase enhanced 3 times the glucose content. The results present enzyme extracts with high yield in saccharification of complex substrates with a potential application in bioethanol production.",

keywords = "cellulases, Fungi, hemicellulases, italique, saccharification, Trichoderma, wheat bran",

author = "Ourdia Bouzid and Amina Allouache and {de Vries}, {Ronald P.} and Dalila Zitouni and Isabelle Benoit-Gelber and Jos Houbraken and Aziza, {Majda Amina}",

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doi = "10.1080/17597269.2023.2295661",

language = "English",

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T1 - High potential low-cost crude enzymes from Algerian fungal strains for plant polysaccharides hydrolysis

AU - Bouzid, Ourdia

AU - Allouache, Amina

AU - de Vries, Ronald P.

AU - Zitouni, Dalila

AU - Benoit-Gelber, Isabelle

AU - Houbraken, Jos

AU - Aziza, Majda Amina

PY - 2024

Y1 - 2024

N2 - Fungi secrete large enzyme sets, such as glycosyl hydrolases (GH) capable of targeting plant polymers, making them a valuable enzymes source. In this study, three fungal strains were isolated from the rhizosphere area and identified as Trichoderma harzianum, Aspergillus tubingensis and Aspergillus tamarii by molecular analysis. The strains were able to grow on mono-, di- and polysaccharides as sole carbon sources, and even on D-galactose and cellulose which is rare for Aspergillus species. Besides, GH activities such as cellobiohydrolase, β-1,4-D-galactosidase, β-mannosidase glucoamylase and β-1,4-D-galactosidase were revealed in secretome of the strains cultivated on wheat bran with optimum obtained between 4th and 7th day. Furthermore, hydrolysis of wheat bran with T. harzianum, A. tubingensis and A. tamarii culture supernatant resulted in the release of 4.6, 5.5 and6.8 g/L of glucose, respectively. The yield increased two-fold by applying a mix of T. harzianum and A. tubingensis enzymes extracts at a ratio 1:1. In addition, the combination of the crude enzymes with commercial cellulase enhanced 3 times the glucose content. The results present enzyme extracts with high yield in saccharification of complex substrates with a potential application in bioethanol production.

AB - Fungi secrete large enzyme sets, such as glycosyl hydrolases (GH) capable of targeting plant polymers, making them a valuable enzymes source. In this study, three fungal strains were isolated from the rhizosphere area and identified as Trichoderma harzianum, Aspergillus tubingensis and Aspergillus tamarii by molecular analysis. The strains were able to grow on mono-, di- and polysaccharides as sole carbon sources, and even on D-galactose and cellulose which is rare for Aspergillus species. Besides, GH activities such as cellobiohydrolase, β-1,4-D-galactosidase, β-mannosidase glucoamylase and β-1,4-D-galactosidase were revealed in secretome of the strains cultivated on wheat bran with optimum obtained between 4th and 7th day. Furthermore, hydrolysis of wheat bran with T. harzianum, A. tubingensis and A. tamarii culture supernatant resulted in the release of 4.6, 5.5 and6.8 g/L of glucose, respectively. The yield increased two-fold by applying a mix of T. harzianum and A. tubingensis enzymes extracts at a ratio 1:1. In addition, the combination of the crude enzymes with commercial cellulase enhanced 3 times the glucose content. The results present enzyme extracts with high yield in saccharification of complex substrates with a potential application in bioethanol production.

KW - cellulases

KW - Fungi

KW - hemicellulases

KW - italique

KW - saccharification

KW - Trichoderma

KW - wheat bran

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DO - 10.1080/17597269.2023.2295661

M3 - Article

AN - SCOPUS:85180200236

SN - 1759-7269

VL - 15

SP - 767

EP - 772

JO - Biofuels

JF - Biofuels

IS - 7

ER -

High potential low-cost crude enzymes from Algerian fungal strains for plant polysaccharides hydrolysis

Abstract

Keywords

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