Identification and molecular characterization of BP75, a novel bromodomain-containing protein

E. Cuppen; M. van Ham; B. Pepers; B. Wieringa; W. Hendriks

doi:10.1016/S0014-5793(99)01191-6

Identification and molecular characterization of BP75, a novel bromodomain-containing protein

E. Cuppen, M. van Ham, B. Pepers, B. Wieringa, W. Hendriks

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

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Abstract

We here describe the identification and characterization of a novel bromodomain-containing protein, the bromodomain protein of 75 kDa (BP75). Initially, we identified BP75 in a two-hybrid screening for proteins that interact with the first PDZ (acronym for post-synaptic density protein PSD-95, Drosophila discs large tumor suppressor DlgA and the tight junction protein ZO-1) domain in protein tyrosine phosphatase-BAS-like (PTP-BL). We found that BP75 is expressed ubiquitously and show that both BP75 and a PTP-BL deletion mutant consisting of the first PDZ domain are located mainly in the nucleus, although cytoplasmic localization is also evident. Full-length PTP-BL, on the contrary, is predominantly localized in the cytoplasm, although some basal nuclear staining is observed. The described molecular interaction may reflect a mechanism of coupling submembraneous signalling events and nuclear events.

Original language	English
Pages (from-to)	291-298
Journal	FEBS Letters
Volume	459
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)01191-6
Publication status	Published - 1999

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title = "Identification and molecular characterization of BP75, a novel bromodomain-containing protein",

abstract = "We here describe the identification and characterization of a novel bromodomain-containing protein, the bromodomain protein of 75 kDa (BP75). Initially, we identified BP75 in a two-hybrid screening for proteins that interact with the first PDZ (acronym for post-synaptic density protein PSD-95, Drosophila discs large tumor suppressor DlgA and the tight junction protein ZO-1) domain in protein tyrosine phosphatase-BAS-like (PTP-BL). We found that BP75 is expressed ubiquitously and show that both BP75 and a PTP-BL deletion mutant consisting of the first PDZ domain are located mainly in the nucleus, although cytoplasmic localization is also evident. Full-length PTP-BL, on the contrary, is predominantly localized in the cytoplasm, although some basal nuclear staining is observed. The described molecular interaction may reflect a mechanism of coupling submembraneous signalling events and nuclear events.",

author = "E. Cuppen and {van Ham}, M. and B. Pepers and B. Wieringa and W. Hendriks",

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doi = "10.1016/S0014-5793(99)01191-6",

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T1 - Identification and molecular characterization of BP75, a novel bromodomain-containing protein

AU - Cuppen, E.

AU - van Ham, M.

AU - Pepers, B.

AU - Wieringa, B.

AU - Hendriks, W.

N1 - Reporting year: 1999 Metis note: 10526152

PY - 1999

Y1 - 1999

N2 - We here describe the identification and characterization of a novel bromodomain-containing protein, the bromodomain protein of 75 kDa (BP75). Initially, we identified BP75 in a two-hybrid screening for proteins that interact with the first PDZ (acronym for post-synaptic density protein PSD-95, Drosophila discs large tumor suppressor DlgA and the tight junction protein ZO-1) domain in protein tyrosine phosphatase-BAS-like (PTP-BL). We found that BP75 is expressed ubiquitously and show that both BP75 and a PTP-BL deletion mutant consisting of the first PDZ domain are located mainly in the nucleus, although cytoplasmic localization is also evident. Full-length PTP-BL, on the contrary, is predominantly localized in the cytoplasm, although some basal nuclear staining is observed. The described molecular interaction may reflect a mechanism of coupling submembraneous signalling events and nuclear events.

AB - We here describe the identification and characterization of a novel bromodomain-containing protein, the bromodomain protein of 75 kDa (BP75). Initially, we identified BP75 in a two-hybrid screening for proteins that interact with the first PDZ (acronym for post-synaptic density protein PSD-95, Drosophila discs large tumor suppressor DlgA and the tight junction protein ZO-1) domain in protein tyrosine phosphatase-BAS-like (PTP-BL). We found that BP75 is expressed ubiquitously and show that both BP75 and a PTP-BL deletion mutant consisting of the first PDZ domain are located mainly in the nucleus, although cytoplasmic localization is also evident. Full-length PTP-BL, on the contrary, is predominantly localized in the cytoplasm, although some basal nuclear staining is observed. The described molecular interaction may reflect a mechanism of coupling submembraneous signalling events and nuclear events.

U2 - 10.1016/S0014-5793(99)01191-6

DO - 10.1016/S0014-5793(99)01191-6

M3 - Article

SN - 0014-5793

VL - 459

SP - 291

EP - 298

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Identification and molecular characterization of BP75, a novel bromodomain-containing protein

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