Identification and molecular characterization of BP75, a novel bromodomain-containing protein

E. Cuppen, M. van Ham, B. Pepers, B. Wieringa, W. Hendriks

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

We here describe the identification and characterization of a novel bromodomain-containing protein, the bromodomain protein of 75 kDa (BP75). Initially, we identified BP75 in a two-hybrid screening for proteins that interact with the first PDZ (acronym for post-synaptic density protein PSD-95, Drosophila discs large tumor suppressor DlgA and the tight junction protein ZO-1) domain in protein tyrosine phosphatase-BAS-like (PTP-BL). We found that BP75 is expressed ubiquitously and show that both BP75 and a PTP-BL deletion mutant consisting of the first PDZ domain are located mainly in the nucleus, although cytoplasmic localization is also evident. Full-length PTP-BL, on the contrary, is predominantly localized in the cytoplasm, although some basal nuclear staining is observed. The described molecular interaction may reflect a mechanism of coupling submembraneous signalling events and nuclear events.
Original languageEnglish
Pages (from-to)291-298
JournalFEBS Letters
Volume459
Issue number3
DOIs
Publication statusPublished - 1999

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