Identification of a discrete subpopulation of spinal cord ependymal cells with neural stem cell properties

Moa Stenudd, Hanna Sabelström, Enric Llorens-Bobadilla, Margherita Zamboni, Hans Blom, Hjalmar Brismar, Shupei Zhang, Onur Basak, Hans Clevers, Christian Göritz, Fanie Barnabé-Heider, Jonas Frisén

Research output: Contribution to journal/periodicalArticleScientificpeer-review

12 Citations (Scopus)


Spinal cord ependymal cells display neural stem cell properties in vitro and generate scar-forming astrocytes and remyelinating oligodendrocytes after injury. We report that ependymal cells are functionally heterogeneous and identify a small subpopulation (8% of ependymal cells and 0.1% of all cells in a spinal cord segment), which we denote ependymal A (EpA) cells, that accounts for the in vitro stem cell potential in the adult spinal cord. After spinal cord injury, EpA cells undergo self-renewing cell division as they give rise to differentiated progeny. Single-cell transcriptome analysis revealed a loss of ependymal cell gene expression programs as EpA cells gained signaling entropy and dedifferentiated to a stem-cell-like transcriptional state after an injury. We conclude that EpA cells are highly differentiated cells that can revert to a stem cell state and constitute a therapeutic target for spinal cord repair.

Original languageEnglish
Pages (from-to)110440
JournalCell Reports
Issue number9
Publication statusPublished - 01 Mar 2022


  • Cell Differentiation/physiology
  • Humans
  • Neural Stem Cells/metabolism
  • Neuroglia
  • Spinal Cord/metabolism
  • Spinal Cord Injuries/metabolism


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