Identification of a male-specific AFLP marker in a functionally dioecious fig, Ficus fulva Reinw. ex Bl. (Moraceae)

TY - JOUR

T1 - Identification of a male-specific AFLP marker in a functionally dioecious fig, Ficus fulva Reinw. ex Bl. (Moraceae)

AU - Parrish, T.L.

AU - Koelewijn, H.P.

AU - van Dijk, P.J.

N1 - Reporting year: 2004 Metis note: 3311; CTE; PVP; file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs2004/Parrish_ea_3311.pdf

PY - 2004

Y1 - 2004

N2 - A male-specific amplified fragment length polymorphism (AFLP) marker was identified in the functionally dioecious fig species, Ficus fulva. A total of 89 polymorphic fragments from three primer combinations were produced, of which one (246 bp) was present in all males (n=23) and absent in all females (n=24) of two populations. This strong association suggests a tight chromosomal linkage between the AFLP marker and the sex-controlling locus. Further analysis indicated that the marker segregated in open-pollinated progenies from natural populations in a 1:1 ratio (n=156), implying that males are the heterogametic sex. Chromosome preparations showed no evidence for morphologically distinct sex chromosomes. The low frequencies of associated markers argue against a morphologically cryptic non-recombining sex chromosome. The sex-locus is therefore likely to be autosomal. The male-specific AFLP marker was sequenced and converted into a sequence characterised amplified region (SCAR) marker. This SCAR marker produced a fragment of equal size in males and females, suggesting that sequence divergence between male- and female-specific chromosomal regions is low. [KEYWORDS: Sex determination ; Functional dioecy ; Ficus ; Heterogametic sex]

AB - A male-specific amplified fragment length polymorphism (AFLP) marker was identified in the functionally dioecious fig species, Ficus fulva. A total of 89 polymorphic fragments from three primer combinations were produced, of which one (246 bp) was present in all males (n=23) and absent in all females (n=24) of two populations. This strong association suggests a tight chromosomal linkage between the AFLP marker and the sex-controlling locus. Further analysis indicated that the marker segregated in open-pollinated progenies from natural populations in a 1:1 ratio (n=156), implying that males are the heterogametic sex. Chromosome preparations showed no evidence for morphologically distinct sex chromosomes. The low frequencies of associated markers argue against a morphologically cryptic non-recombining sex chromosome. The sex-locus is therefore likely to be autosomal. The male-specific AFLP marker was sequenced and converted into a sequence characterised amplified region (SCAR) marker. This SCAR marker produced a fragment of equal size in males and females, suggesting that sequence divergence between male- and female-specific chromosomal regions is low. [KEYWORDS: Sex determination ; Functional dioecy ; Ficus ; Heterogametic sex]

KW - NIOO/CTE/PVP

U2 - 10.1007/s00497-004-0208-x

DO - 10.1007/s00497-004-0208-x

M3 - Article

SN - 0934-0882

VL - 17

SP - 17

EP - 22

JO - Sexual Plant Reproduction

JF - Sexual Plant Reproduction

IS - 1

ER -