If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer

L. Bonapace; J. Wyckoff; T. Oertner; J. van Rheenen; T. Junt; M. Bentires-Alj

doi:10.1007/s10911-012-9250-8

If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer

L. Bonapace, J. Wyckoff, T. Oertner, J. van Rheenen, T. Junt, M. Bentires-Alj

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

17 Citations (Scopus)

Abstract

A fundamental hallmark of cancer is progression to metastasis and the growth of breast cancer metastases in lung, bone, liver and/or brain causes fatal complications. Unfortunately, the cellular and biochemical mechanisms of the metastatic process remain ill-defined. Recent application of intravital multiphoton microscopy (MP-IVM) to image fluorescently labeled cells in mouse models of cancer has allowed dynamic observation of this multi-step process at the cellular and subcellular levels. In this article, we discuss the use of MP-IVM in studies of breast cancer metastasis, as well as surgical techniques for exposing tumors prior to imaging. We also describe a versatile multiphoton microscope for imaging tumor-stroma interactions.

Original language	English
Pages (from-to)	125-129
Journal	Journal of Mammary Gland Biology and Neoplasia
Volume	17
Issue number	2
DOIs	https://doi.org/10.1007/s10911-012-9250-8
Publication status	Published - 2012

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title = "If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer",

abstract = "A fundamental hallmark of cancer is progression to metastasis and the growth of breast cancer metastases in lung, bone, liver and/or brain causes fatal complications. Unfortunately, the cellular and biochemical mechanisms of the metastatic process remain ill-defined. Recent application of intravital multiphoton microscopy (MP-IVM) to image fluorescently labeled cells in mouse models of cancer has allowed dynamic observation of this multi-step process at the cellular and subcellular levels. In this article, we discuss the use of MP-IVM in studies of breast cancer metastasis, as well as surgical techniques for exposing tumors prior to imaging. We also describe a versatile multiphoton microscope for imaging tumor-stroma interactions.",

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AU - Bonapace, L.

AU - Wyckoff, J.

AU - Oertner, T.

AU - van Rheenen, J.

AU - Junt, T.

AU - Bentires-Alj, M.

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AB - A fundamental hallmark of cancer is progression to metastasis and the growth of breast cancer metastases in lung, bone, liver and/or brain causes fatal complications. Unfortunately, the cellular and biochemical mechanisms of the metastatic process remain ill-defined. Recent application of intravital multiphoton microscopy (MP-IVM) to image fluorescently labeled cells in mouse models of cancer has allowed dynamic observation of this multi-step process at the cellular and subcellular levels. In this article, we discuss the use of MP-IVM in studies of breast cancer metastasis, as well as surgical techniques for exposing tumors prior to imaging. We also describe a versatile multiphoton microscope for imaging tumor-stroma interactions.

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DO - 10.1007/s10911-012-9250-8

M3 - Article

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JO - Journal of Mammary Gland Biology and Neoplasia

JF - Journal of Mammary Gland Biology and Neoplasia

IS - 2

ER -

If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer

Abstract

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