Imaging Translation Dynamics of Single mRNA Molecules in Live Cells

Suzan Ruijtenberg, Tim A Hoek, Xiaowei Yan, Marvin E Tanenbaum

Research output: Contribution to journal/periodicalArticleScientificpeer-review

7 Citations (Scopus)


mRNA translation is a key step in decoding the genetic information stored in DNA. Regulation of translation efficiency contributes to gene expression control and is therefore important for cell fate and function. Here, we describe a recently developed microscopy-based method that allows for visualization of translation of single mRNAs in live cells. The ability to measure translation dynamics of single mRNAs will enable a better understanding of spatiotemporal control of translation, and will provide unique insights into translational heterogeneity of different mRNA molecules in single cells.

Original languageEnglish
Pages (from-to)385-404
Number of pages20
JournalMethods in Molecular Biology
Publication statusPublished - 2018


  • Cell Line
  • Cell Survival
  • Fluorescence
  • Green Fluorescent Proteins/metabolism
  • Humans
  • Image Processing, Computer-Assisted
  • Protein Biosynthesis
  • RNA, Messenger/genetics
  • Ribosomes/metabolism
  • Single Molecule Imaging/methods
  • Single-Chain Antibodies/metabolism


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