TY - JOUR
T1 - Impact of parallel fiber to Purkinje cell long-term depression is unmasked in absence of inhibitory input.
AU - Boele, Henk-Jan
AU - Peter, Saša
AU - Ten Brinke, Michiel M
AU - Verdonschot, Lucas
AU - IJpelaar, Anna C H
AU - Rizopoulos, Dimitris
AU - Gao, Zhenyu
AU - Koekkoek, Sebastiaan K E
AU - De Zeeuw, Chris I
PY - 2018/10
Y1 - 2018/10
N2 - Pavlovian eyeblink conditioning has been used extensively to study the neural mechanisms underlying associative and motor learning. During this simple learning task, memory formation takes place at Purkinje cells in defined areas of the cerebellar cortex, which acquire a strong temporary suppression of their activity during conditioning. Yet, it is unknown which neuronal plasticity mechanisms mediate this suppression. Two potential mechanisms include long-term depression of parallel fiber to Purkinje cell synapses and feed-forward inhibition by molecular layer interneurons. We show, using a triple transgenic approach, that only concurrent disruption of both these suppression mechanisms can severely impair conditioning, highlighting that both processes can compensate for each other's deficits.
AB - Pavlovian eyeblink conditioning has been used extensively to study the neural mechanisms underlying associative and motor learning. During this simple learning task, memory formation takes place at Purkinje cells in defined areas of the cerebellar cortex, which acquire a strong temporary suppression of their activity during conditioning. Yet, it is unknown which neuronal plasticity mechanisms mediate this suppression. Two potential mechanisms include long-term depression of parallel fiber to Purkinje cell synapses and feed-forward inhibition by molecular layer interneurons. We show, using a triple transgenic approach, that only concurrent disruption of both these suppression mechanisms can severely impair conditioning, highlighting that both processes can compensate for each other's deficits.
U2 - 10.1126/sciadv.aas9426
DO - 10.1126/sciadv.aas9426
M3 - Article
C2 - 30306129
VL - 4
JO - Science advances
JF - Science advances
IS - 10
M1 - eaas9426
ER -