Improved genetic manipulation of human embryonic stem cells.

S.R. Braam; C. Denning; S. van den Brink; P. Kats; R. Hochstenbach; R. Passier; C.L. Mummery

doi:10.1038/nmeth.1200

Improved genetic manipulation of human embryonic stem cells.

S.R. Braam, C. Denning, S. van den Brink, P. Kats, R. Hochstenbach, R. Passier, C.L. Mummery

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

87 Citations (Scopus)

Abstract

Low efficiency of transfection limits the ability to genetically manipulate human embryonic stem cells (hESCs), and differences in cell derivation and culture methods require optimization of transfection protocols. We transiently transferred multiple independent hESC lines with different growth requirements to standardized feeder-free culture, and optimized conditions for clonal growth and efficient gene transfer without loss of pluripotency. Stably transfected lines retained differentiation potential, and most lines displayed normal karyotypes.

Original language	English
Pages (from-to)	389-392
Journal	Nature Methods
Volume	5
Issue number	5
DOIs	https://doi.org/10.1038/nmeth.1200
Publication status	Published - 2008

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10.1038/nmeth.1200

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title = "Improved genetic manipulation of human embryonic stem cells.",

abstract = "Low efficiency of transfection limits the ability to genetically manipulate human embryonic stem cells (hESCs), and differences in cell derivation and culture methods require optimization of transfection protocols. We transiently transferred multiple independent hESC lines with different growth requirements to standardized feeder-free culture, and optimized conditions for clonal growth and efficient gene transfer without loss of pluripotency. Stably transfected lines retained differentiation potential, and most lines displayed normal karyotypes.",

author = "S.R. Braam and C. Denning and {van den Brink}, S. and P. Kats and R. Hochstenbach and R. Passier and C.L. Mummery",

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AU - Braam, S.R.

AU - Denning, C.

AU - van den Brink, S.

AU - Kats, P.

AU - Hochstenbach, R.

AU - Passier, R.

AU - Mummery, C.L.

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PY - 2008

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AB - Low efficiency of transfection limits the ability to genetically manipulate human embryonic stem cells (hESCs), and differences in cell derivation and culture methods require optimization of transfection protocols. We transiently transferred multiple independent hESC lines with different growth requirements to standardized feeder-free culture, and optimized conditions for clonal growth and efficient gene transfer without loss of pluripotency. Stably transfected lines retained differentiation potential, and most lines displayed normal karyotypes.

U2 - 10.1038/nmeth.1200

DO - 10.1038/nmeth.1200

M3 - Article

SN - 1548-7091

VL - 5

SP - 389

EP - 392

JO - Nature Methods

JF - Nature Methods

IS - 5

ER -

Improved genetic manipulation of human embryonic stem cells.

Abstract

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