In situ hybridization to identify gut stem cells

A. Gregorieff, H. Clevers

Research output: Contribution to journal/periodicalArticleScientificpeer-review

33 Citations (Scopus)


In recent years, considerable effort has been directed towards identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.
Original languageEnglish
Pages (from-to)1
JournalCurrent Protocols in Stem Cell Biology
VolumeChapter 2
Publication statusPublished - 2010


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