In vivo generation of haematopoietic stem/progenitor cells from bone marrow-derived haemogenic endothelium

TY - JOUR

T1 - In vivo generation of haematopoietic stem/progenitor cells from bone marrow-derived haemogenic endothelium

AU - Yvernogeau, Laurent

AU - Gautier, Rodolphe

AU - Petit, Laurence

AU - Khoury, Hanane

AU - Relaix, Frédéric

AU - Ribes, Vanessa

AU - Sang, Helen

AU - Charbord, Pierre

AU - Souyri, Michèle

AU - Robin, Catherine

AU - Jaffredo, Thierry

PY - 2019/11

Y1 - 2019/11

N2 - It is well established that haematopoietic stem and progenitor cells (HSPCs) are generated from a transient subset of specialized endothelial cells termed haemogenic, present in the yolk sac, placenta and aorta, through an endothelial-to-haematopoietic transition (EHT). HSPC generation via EHT is thought to be restricted to the early stages of development. By using experimental embryology and genetic approaches in birds and mice, respectively, we document here the discovery of a bone marrow haemogenic endothelium in the late fetus/young adult. These cells are capable of de novo producing a cohort of HSPCs in situ that harbour a very specific molecular signature close to that of aortic endothelial cells undergoing EHT or their immediate progenies, i.e., recently emerged HSPCs. Taken together, our results reveal that HSPCs can be generated de novo past embryonic stages. Understanding the molecular events controlling this production will be critical for devising innovative therapies.

AB - It is well established that haematopoietic stem and progenitor cells (HSPCs) are generated from a transient subset of specialized endothelial cells termed haemogenic, present in the yolk sac, placenta and aorta, through an endothelial-to-haematopoietic transition (EHT). HSPC generation via EHT is thought to be restricted to the early stages of development. By using experimental embryology and genetic approaches in birds and mice, respectively, we document here the discovery of a bone marrow haemogenic endothelium in the late fetus/young adult. These cells are capable of de novo producing a cohort of HSPCs in situ that harbour a very specific molecular signature close to that of aortic endothelial cells undergoing EHT or their immediate progenies, i.e., recently emerged HSPCs. Taken together, our results reveal that HSPCs can be generated de novo past embryonic stages. Understanding the molecular events controlling this production will be critical for devising innovative therapies.

U2 - 10.1038/s41556-019-0410-6

DO - 10.1038/s41556-019-0410-6

M3 - Article

C2 - 31685991

SN - 1465-7392

VL - 21

SP - 1334

EP - 1345

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 11

ER -