In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique

TY - JOUR

T1 - In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique

AU - Ritsma, L.

AU - Vrisekoop, N.

AU - van Rheenen, J.

N1 - Reporting year: 2013

PY - 2013

Y1 - 2013

N2 - Intravital microscopy has been used extensively to study dynamic processes in the context of a living animal; however, only a limited number of fluorescent probes and mouse models are available. By contrast, many dyes and antibodies exist for the immuno-labelling of fixed tissue. Here we report a method that combines the advantages of histochemistry and in vivo imaging by correlating cryosection labelling with corresponding intravital microscopy images (CLIM). Using CLIM, we find that the presence of CD3(+) T cells correlates with mammary tumour cell migration. When CD4(+) and CD8(+) T-cell subsets are depleted, reduced tumour cell migration is observed. From these data we conclude that CLIM is a powerful tool to correlate intravital microscopy data with cryosection labelling data.

AB - Intravital microscopy has been used extensively to study dynamic processes in the context of a living animal; however, only a limited number of fluorescent probes and mouse models are available. By contrast, many dyes and antibodies exist for the immuno-labelling of fixed tissue. Here we report a method that combines the advantages of histochemistry and in vivo imaging by correlating cryosection labelling with corresponding intravital microscopy images (CLIM). Using CLIM, we find that the presence of CD3(+) T cells correlates with mammary tumour cell migration. When CD4(+) and CD8(+) T-cell subsets are depleted, reduced tumour cell migration is observed. From these data we conclude that CLIM is a powerful tool to correlate intravital microscopy data with cryosection labelling data.

U2 - 10.1038/ncomms3366

DO - 10.1038/ncomms3366

M3 - Article

SN - 2041-1723

VL - 4

SP - 2366

JO - Nature Communications

JF - Nature Communications

ER -