In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique

L. Ritsma; N. Vrisekoop; J. van Rheenen

doi:10.1038/ncomms3366

In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique

L. Ritsma, N. Vrisekoop, J. van Rheenen

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

Intravital microscopy has been used extensively to study dynamic processes in the context of a living animal; however, only a limited number of fluorescent probes and mouse models are available. By contrast, many dyes and antibodies exist for the immuno-labelling of fixed tissue. Here we report a method that combines the advantages of histochemistry and in vivo imaging by correlating cryosection labelling with corresponding intravital microscopy images (CLIM). Using CLIM, we find that the presence of CD3(+) T cells correlates with mammary tumour cell migration. When CD4(+) and CD8(+) T-cell subsets are depleted, reduced tumour cell migration is observed. From these data we conclude that CLIM is a powerful tool to correlate intravital microscopy data with cryosection labelling data.

Original language	English
Pages (from-to)	2366
Journal	Nature Communications
Volume	4
DOIs	https://doi.org/10.1038/ncomms3366
Publication status	Published - 2013

Access to Document

10.1038/ncomms3366

Cite this

@article{8377aaccbd94435ea8dff7453268aed1,

title = "In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique",

abstract = "Intravital microscopy has been used extensively to study dynamic processes in the context of a living animal; however, only a limited number of fluorescent probes and mouse models are available. By contrast, many dyes and antibodies exist for the immuno-labelling of fixed tissue. Here we report a method that combines the advantages of histochemistry and in vivo imaging by correlating cryosection labelling with corresponding intravital microscopy images (CLIM). Using CLIM, we find that the presence of CD3(+) T cells correlates with mammary tumour cell migration. When CD4(+) and CD8(+) T-cell subsets are depleted, reduced tumour cell migration is observed. From these data we conclude that CLIM is a powerful tool to correlate intravital microscopy data with cryosection labelling data.",

author = "L. Ritsma and N. Vrisekoop and {van Rheenen}, J.",

note = "Reporting year: 2013",

year = "2013",

doi = "10.1038/ncomms3366",

language = "English",

volume = "4",

pages = "2366",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique

AU - Ritsma, L.

AU - Vrisekoop, N.

AU - van Rheenen, J.

N1 - Reporting year: 2013

PY - 2013

Y1 - 2013

N2 - Intravital microscopy has been used extensively to study dynamic processes in the context of a living animal; however, only a limited number of fluorescent probes and mouse models are available. By contrast, many dyes and antibodies exist for the immuno-labelling of fixed tissue. Here we report a method that combines the advantages of histochemistry and in vivo imaging by correlating cryosection labelling with corresponding intravital microscopy images (CLIM). Using CLIM, we find that the presence of CD3(+) T cells correlates with mammary tumour cell migration. When CD4(+) and CD8(+) T-cell subsets are depleted, reduced tumour cell migration is observed. From these data we conclude that CLIM is a powerful tool to correlate intravital microscopy data with cryosection labelling data.

AB - Intravital microscopy has been used extensively to study dynamic processes in the context of a living animal; however, only a limited number of fluorescent probes and mouse models are available. By contrast, many dyes and antibodies exist for the immuno-labelling of fixed tissue. Here we report a method that combines the advantages of histochemistry and in vivo imaging by correlating cryosection labelling with corresponding intravital microscopy images (CLIM). Using CLIM, we find that the presence of CD3(+) T cells correlates with mammary tumour cell migration. When CD4(+) and CD8(+) T-cell subsets are depleted, reduced tumour cell migration is observed. From these data we conclude that CLIM is a powerful tool to correlate intravital microscopy data with cryosection labelling data.

U2 - 10.1038/ncomms3366

DO - 10.1038/ncomms3366

M3 - Article

SN - 2041-1723

VL - 4

SP - 2366

JO - Nature Communications

JF - Nature Communications

ER -

In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique

Abstract

Access to Document

Fingerprint

Cite this