Inhibitory interneuron classes express complementary AMPA-receptor patterns in macaque primary visual cortex

TY - JOUR

T1 - Inhibitory interneuron classes express complementary AMPA-receptor patterns in macaque primary visual cortex

AU - Kooijmans, Roxana N

AU - Self, Matthew W

AU - Wouterlood, Floris G

AU - Beliën, Jeroen A M

AU - Roelfsema, Pieter R

PY - 2014/4/30

Y1 - 2014/4/30

N2 - Glutamate receptors mediate excitatory neurotransmission. A very prevalent type of glutamate receptor in the neocortex is the AMPA receptor (AMPAR). AMPARs mediate fast synaptic transmission and their functionality depends on the subunit composition. In primary visual cortex (area V1), the density and subunit composition of AMPARs differ among cortical layers and among cell types. The AMPARs expressed by the different types of inhibitory interneurons, which are crucial for network function, have not yet been characterized systematically. We investigated the distribution of AMPAR subunits in macaque V1 for three distinct subpopulations of inhibitory interneurons: parvalbumin-immunoreactive (PV-IR) interneurons, calbindin-immunoreactive (CB-IR) interneurons, and calretinin-immunoreactive (CR-IR) interneurons. We found that PV-IR cells, which have previously been identified as fast spiking, show high expression of the GluA2 and GluA3 subunits. In contrast, CB-IR and CR-IR cells, which tend to be intermediate spiking, show high expression of the GluA1 and GluA4 subunits. Thus, our data demonstrate that the expression of AMPARs divides inhibitory interneurons in macaque V1 into two categories that are compatible with existing classification methods based on calcium-binding proteins and firing behavior. Moreover, our findings suggest new approaches to target the different inhibitory interneuron classes pharmacologically in vivo.

AB - Glutamate receptors mediate excitatory neurotransmission. A very prevalent type of glutamate receptor in the neocortex is the AMPA receptor (AMPAR). AMPARs mediate fast synaptic transmission and their functionality depends on the subunit composition. In primary visual cortex (area V1), the density and subunit composition of AMPARs differ among cortical layers and among cell types. The AMPARs expressed by the different types of inhibitory interneurons, which are crucial for network function, have not yet been characterized systematically. We investigated the distribution of AMPAR subunits in macaque V1 for three distinct subpopulations of inhibitory interneurons: parvalbumin-immunoreactive (PV-IR) interneurons, calbindin-immunoreactive (CB-IR) interneurons, and calretinin-immunoreactive (CR-IR) interneurons. We found that PV-IR cells, which have previously been identified as fast spiking, show high expression of the GluA2 and GluA3 subunits. In contrast, CB-IR and CR-IR cells, which tend to be intermediate spiking, show high expression of the GluA1 and GluA4 subunits. Thus, our data demonstrate that the expression of AMPARs divides inhibitory interneurons in macaque V1 into two categories that are compatible with existing classification methods based on calcium-binding proteins and firing behavior. Moreover, our findings suggest new approaches to target the different inhibitory interneuron classes pharmacologically in vivo.

KW - Animals

KW - Calbindin 2

KW - Calbindins

KW - Interneurons

KW - Macaca mulatta

KW - Male

KW - Neural Inhibition

KW - Parvalbumins

KW - Protein Subunits

KW - Receptors, AMPA

KW - Visual Cortex

U2 - 10.1523/JNEUROSCI.3188-13.2014

DO - 10.1523/JNEUROSCI.3188-13.2014

M3 - Article

C2 - 24790201

SN - 0270-6474

VL - 34

SP - 6303

EP - 6315

JO - Journal of Neuroscience

JF - Journal of Neuroscience

IS - 18

ER -