Inhibitory interneuron classes express complementary AMPA-receptor patterns in macaque primary visual cortex

Roxana N Kooijmans, Matthew W Self, Floris G Wouterlood, Jeroen A M Beliën, Pieter R Roelfsema

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Abstract

Glutamate receptors mediate excitatory neurotransmission. A very prevalent type of glutamate receptor in the neocortex is the AMPA receptor (AMPAR). AMPARs mediate fast synaptic transmission and their functionality depends on the subunit composition. In primary visual cortex (area V1), the density and subunit composition of AMPARs differ among cortical layers and among cell types. The AMPARs expressed by the different types of inhibitory interneurons, which are crucial for network function, have not yet been characterized systematically. We investigated the distribution of AMPAR subunits in macaque V1 for three distinct subpopulations of inhibitory interneurons: parvalbumin-immunoreactive (PV-IR) interneurons, calbindin-immunoreactive (CB-IR) interneurons, and calretinin-immunoreactive (CR-IR) interneurons. We found that PV-IR cells, which have previously been identified as fast spiking, show high expression of the GluA2 and GluA3 subunits. In contrast, CB-IR and CR-IR cells, which tend to be intermediate spiking, show high expression of the GluA1 and GluA4 subunits. Thus, our data demonstrate that the expression of AMPARs divides inhibitory interneurons in macaque V1 into two categories that are compatible with existing classification methods based on calcium-binding proteins and firing behavior. Moreover, our findings suggest new approaches to target the different inhibitory interneuron classes pharmacologically in vivo.

Original languageEnglish
Pages (from-to)6303-15
Number of pages13
JournalJournal of Neuroscience
Volume34
Issue number18
DOIs
Publication statusPublished - 30 Apr 2014

Keywords

  • Animals
  • Calbindin 2
  • Calbindins
  • Interneurons
  • Macaca mulatta
  • Male
  • Neural Inhibition
  • Parvalbumins
  • Protein Subunits
  • Receptors, AMPA
  • Visual Cortex

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