Documents

DOI

  • Pascale Flury
  • Nora Aellen
  • Beat Ruffner
  • Maria Pechy-Tarr
  • Shakira Fataar
  • Zane Metla
  • Ana Dominguez-Ferreras
  • Guido Bloemberg
  • Joachim Frey
  • Alexander Goesmann
  • Jos M Raaijmakers
  • Brion Duffy
  • Monica Hofte
  • Jochen Blom
  • Theo H M Smits
  • Christoph Keel
  • Monika Maurhofer
Bacteria of the genus Pseudomonas occupy diverse environments. The Pseudomonas fluorescens group is particularly well-known for its plant-beneficial properties including pathogen suppression. Recent observations that some strains of this group also cause lethal infections in insect larvae, however, point to a more versatile ecology of these bacteria. We show that 26 P. fluorescens group strains, isolated from three continents and covering three phylogenetically distinct sub-clades, exhibited different activities toward lepidopteran larvae, ranging from lethal to avirulent. All strains of sub-clade 1, which includes Pseudomonas chlororaphis and Pseudomonas protegens, were highly insecticidal regardless of their origin (animals, plants). Comparative genomics revealed that strains in this sub-clade possess specific traits allowing a switch between plant- and insect-associated lifestyles. We identified 90 genes unique to all highly insecticidal strains (sub-clade 1) and 117 genes common to all strains of sub-clade 1 and present in some moderately insecticidal strains of sub-clade 3. Mutational analysis of selected genes revealed the importance of chitinase C and phospholipase C in insect pathogenicity. The study provides insight into the genetic basis and phylogenetic distribution of traits defining insecticidal activity in plant-beneficial pseudomonads. Strains with potent dual activity against plant pathogens and herbivorous insects have great potential for use in integrated pest management for crops.
Original languageEnglish
Pages (from-to)2527-2542
JournalISME Journal
Volume10
Early online date19 Feb 2016
DOI
Publication statusPublished - 2016

    Research areas

  • international

ID: 1793526