Inter- and intracellular colonization of Arabidopsis roots by endophytic actinobacteria and the impact of plant hormones on their antimicrobial activity

Anne Van der Meij; Joost Willemse; Martinus A. Schneijderberg; Rene Geurts; Jos Raaijmakers; Gilles van Wezel

doi:10.1101/222844

Inter- and intracellular colonization of Arabidopsis roots by endophytic actinobacteria and the impact of plant hormones on their antimicrobial activity

Anne Van der Meij, Joost Willemse, Martinus A. Schneijderberg, Rene Geurts, Jos Raaijmakers, Gilles van Wezel (Corresponding author)

NIOO - Microbial Ecology (ME)

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Abstract

Many actinobacteria live in close association with eukaryotes like fungi, insects, animals and plants. Plant-associated actinobacteria display (endo)symbiotic, saprophytic or pathogenic life styles, and can make up a substantial part of the endophytic community. Here, we characterised endophytic actinobacteria isolated from root tissue of Arabidopsis thaliana (Arabidopsis) plants grown in soil from a natural ecosystem. Many of these actinobacteria belong to the family of Streptomycetaceae with Streptomyces olivochromogenes and Streptomyces clavifer as well represented species. When seeds of Arabidopsis were inoculated with spores of Streptomyces strain coa1, which shows high similarity to S. olivochromogenes, roots were colonised intercellularly and, unexpectedly, also intracellularly. Subsequent exposure of endophytic isolates to plant hormones typically found in root and shoot tissues of Arabidopsis led to altered antibiotic production against Escherichia coli and Bacillus subtilis. Taken together, our work reveals remarkable colonization patterns of endophytic streptomycetes with specific traits that may allow a competitive advantage inside root tissue.

Original language	English
Pages (from-to)	679-690
Journal	Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Volume	111
Issue number	5
Early online date	15 Jan 2018
DOIs	https://doi.org/10.1101/222844 https://doi.org/10.1007/s10482-018-1014-z
Publication status	Published - 2018

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national

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10.1007/s10482-018-1014-zLicence: CC BY

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Van der Meij, A., Willemse, J., Schneijderberg, M. A., Geurts, R., Raaijmakers, J., & van Wezel, G. (2018). Inter- and intracellular colonization of Arabidopsis roots by endophytic actinobacteria and the impact of plant hormones on their antimicrobial activity. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 111(5), 679-690. https://doi.org/10.1101/222844, https://doi.org/10.1007/s10482-018-1014-z

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abstract = "Many actinobacteria live in close association with eukaryotes like fungi, insects, animals and plants. Plant-associated actinobacteria display (endo)symbiotic, saprophytic or pathogenic life styles, and can make up a substantial part of the endophytic community. Here, we characterised endophytic actinobacteria isolated from root tissue of Arabidopsis thaliana (Arabidopsis) plants grown in soil from a natural ecosystem. Many of these actinobacteria belong to the family of Streptomycetaceae with Streptomyces olivochromogenes and Streptomyces clavifer as well represented species. When seeds of Arabidopsis were inoculated with spores of Streptomyces strain coa1, which shows high similarity to S. olivochromogenes, roots were colonised intercellularly and, unexpectedly, also intracellularly. Subsequent exposure of endophytic isolates to plant hormones typically found in root and shoot tissues of Arabidopsis led to altered antibiotic production against Escherichia coli and Bacillus subtilis. Taken together, our work reveals remarkable colonization patterns of endophytic streptomycetes with specific traits that may allow a competitive advantage inside root tissue.",

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Van der Meij, A, Willemse, J, Schneijderberg, MA, Geurts, R, Raaijmakers, J & van Wezel, G 2018, 'Inter- and intracellular colonization of Arabidopsis roots by endophytic actinobacteria and the impact of plant hormones on their antimicrobial activity', Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, vol. 111, no. 5, pp. 679-690. https://doi.org/10.1101/222844, https://doi.org/10.1007/s10482-018-1014-z

Inter- and intracellular colonization of Arabidopsis roots by endophytic actinobacteria and the impact of plant hormones on their antimicrobial activity. / Van der Meij, Anne; Willemse, Joost; Schneijderberg, Martinus A. et al.
In: Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, Vol. 111, No. 5, 2018, p. 679-690.

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

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AU - Van der Meij, Anne

AU - Willemse, Joost

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AU - Geurts, Rene

AU - Raaijmakers, Jos

AU - van Wezel, Gilles

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AB - Many actinobacteria live in close association with eukaryotes like fungi, insects, animals and plants. Plant-associated actinobacteria display (endo)symbiotic, saprophytic or pathogenic life styles, and can make up a substantial part of the endophytic community. Here, we characterised endophytic actinobacteria isolated from root tissue of Arabidopsis thaliana (Arabidopsis) plants grown in soil from a natural ecosystem. Many of these actinobacteria belong to the family of Streptomycetaceae with Streptomyces olivochromogenes and Streptomyces clavifer as well represented species. When seeds of Arabidopsis were inoculated with spores of Streptomyces strain coa1, which shows high similarity to S. olivochromogenes, roots were colonised intercellularly and, unexpectedly, also intracellularly. Subsequent exposure of endophytic isolates to plant hormones typically found in root and shoot tissues of Arabidopsis led to altered antibiotic production against Escherichia coli and Bacillus subtilis. Taken together, our work reveals remarkable colonization patterns of endophytic streptomycetes with specific traits that may allow a competitive advantage inside root tissue.

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Van der Meij A, Willemse J, Schneijderberg MA, Geurts R, Raaijmakers J , van Wezel G. Inter- and intracellular colonization of Arabidopsis roots by endophytic actinobacteria and the impact of plant hormones on their antimicrobial activity. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2018;111(5):679-690. Epub 2018 Jan 15. doi: 10.1101/222844, 10.1007/s10482-018-1014-z

Inter- and intracellular colonization of Arabidopsis roots by endophytic actinobacteria and the impact of plant hormones on their antimicrobial activity

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