Interactions between Cyanobacteria and Methane Processing Microbes Mitigate Methane Emissions from Rice Soils

Germán Pérez; Sascha M. B. Krause; Paul L. E. Bodelier; Marion Meima-Franke; Leonardo Pitombo; Pilar Irisarri

doi:10.3390/microorganisms11122830

Interactions between Cyanobacteria and Methane Processing Microbes Mitigate Methane Emissions from Rice Soils

Germán Pérez, Sascha M. B. Krause, Paul L. E. Bodelier^* (Corresponding author), Marion Meima-Franke, Leonardo Pitombo, Pilar Irisarri

^*Corresponding author for this work

NIOO - Microbial Ecology (ME)

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

Cyanobacteria play a relevant role in rice soils due to their contribution to soil fertility through nitrogen (N2) fixation and as a promising strategy to mitigate methane (CH4) emissions from these systems. However, information is still limited regarding the mechanisms of cyanobacterial modulation of CH4 cycling in rice soils. Here, we focused on the response of methane cycling microbial communities to inoculation with cyanobacteria in rice soils. We performed a microcosm study comprising rice soil inoculated with either of two cyanobacterial isolates (Calothrix sp. and Nostoc sp.) obtained from a rice paddy. Our results demonstrate that cyanobacterial inoculation reduced CH4 emissions by 20 times. Yet, the effect on CH4 cycling microbes differed for the cyanobacterial strains. Type Ia methanotrophs were stimulated by Calothrix sp. in the surface layer, while Nostoc sp. had the opposite effect. The overall pmoA transcripts of Type Ib methanotrophs were stimulated by Nostoc. Methanogens were not affected in the surface layer, while their abundance was reduced in the sub surface layer by the presence of Nostoc sp. Our results indicate that mitigation of methane emission from rice soils based on cyanobacterial inoculants depends on the proper pairing of cyanobacteria–methanotrophs and their respective traits.

Original language	English
Article number	2830
Journal	Microorganisms
Volume	11
Issue number	12
DOIs	https://doi.org/10.3390/microorganisms11122830
Publication status	Published - 21 Nov 2023

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10.3390/microorganisms11122830Licence: CC BY

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@article{92afbd59edb24b0695746fc7578aaea6,

title = "Interactions between Cyanobacteria and Methane Processing Microbes Mitigate Methane Emissions from Rice Soils",

abstract = "Cyanobacteria play a relevant role in rice soils due to their contribution to soil fertility through nitrogen (N2) fixation and as a promising strategy to mitigate methane (CH4) emissions from these systems. However, information is still limited regarding the mechanisms of cyanobacterial modulation of CH4 cycling in rice soils. Here, we focused on the response of methane cycling microbial communities to inoculation with cyanobacteria in rice soils. We performed a microcosm study comprising rice soil inoculated with either of two cyanobacterial isolates (Calothrix sp. and Nostoc sp.) obtained from a rice paddy. Our results demonstrate that cyanobacterial inoculation reduced CH4 emissions by 20 times. Yet, the effect on CH4 cycling microbes differed for the cyanobacterial strains. Type Ia methanotrophs were stimulated by Calothrix sp. in the surface layer, while Nostoc sp. had the opposite effect. The overall pmoA transcripts of Type Ib methanotrophs were stimulated by Nostoc. Methanogens were not affected in the surface layer, while their abundance was reduced in the sub surface layer by the presence of Nostoc sp. Our results indicate that mitigation of methane emission from rice soils based on cyanobacterial inoculants depends on the proper pairing of cyanobacteria–methanotrophs and their respective traits.",

author = "Germ{\'a}n P{\'e}rez and Krause, {Sascha M. B.} and Bodelier, {Paul L. E.} and Marion Meima-Franke and Leonardo Pitombo and Pilar Irisarri",

note = "Data archiving: NIOO data, will be made available by Germ{\'a}n.",

year = "2023",

month = nov,

day = "21",

doi = "10.3390/microorganisms11122830",

language = "English",

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journal = "Microorganisms",

issn = "2076-2607",

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T1 - Interactions between Cyanobacteria and Methane Processing Microbes Mitigate Methane Emissions from Rice Soils

AU - Pérez, Germán

AU - Krause, Sascha M. B.

AU - Bodelier, Paul L. E.

AU - Meima-Franke, Marion

AU - Pitombo, Leonardo

AU - Irisarri, Pilar

N1 - Data archiving: NIOO data, will be made available by Germán.

PY - 2023/11/21

Y1 - 2023/11/21

N2 - Cyanobacteria play a relevant role in rice soils due to their contribution to soil fertility through nitrogen (N2) fixation and as a promising strategy to mitigate methane (CH4) emissions from these systems. However, information is still limited regarding the mechanisms of cyanobacterial modulation of CH4 cycling in rice soils. Here, we focused on the response of methane cycling microbial communities to inoculation with cyanobacteria in rice soils. We performed a microcosm study comprising rice soil inoculated with either of two cyanobacterial isolates (Calothrix sp. and Nostoc sp.) obtained from a rice paddy. Our results demonstrate that cyanobacterial inoculation reduced CH4 emissions by 20 times. Yet, the effect on CH4 cycling microbes differed for the cyanobacterial strains. Type Ia methanotrophs were stimulated by Calothrix sp. in the surface layer, while Nostoc sp. had the opposite effect. The overall pmoA transcripts of Type Ib methanotrophs were stimulated by Nostoc. Methanogens were not affected in the surface layer, while their abundance was reduced in the sub surface layer by the presence of Nostoc sp. Our results indicate that mitigation of methane emission from rice soils based on cyanobacterial inoculants depends on the proper pairing of cyanobacteria–methanotrophs and their respective traits.

AB - Cyanobacteria play a relevant role in rice soils due to their contribution to soil fertility through nitrogen (N2) fixation and as a promising strategy to mitigate methane (CH4) emissions from these systems. However, information is still limited regarding the mechanisms of cyanobacterial modulation of CH4 cycling in rice soils. Here, we focused on the response of methane cycling microbial communities to inoculation with cyanobacteria in rice soils. We performed a microcosm study comprising rice soil inoculated with either of two cyanobacterial isolates (Calothrix sp. and Nostoc sp.) obtained from a rice paddy. Our results demonstrate that cyanobacterial inoculation reduced CH4 emissions by 20 times. Yet, the effect on CH4 cycling microbes differed for the cyanobacterial strains. Type Ia methanotrophs were stimulated by Calothrix sp. in the surface layer, while Nostoc sp. had the opposite effect. The overall pmoA transcripts of Type Ib methanotrophs were stimulated by Nostoc. Methanogens were not affected in the surface layer, while their abundance was reduced in the sub surface layer by the presence of Nostoc sp. Our results indicate that mitigation of methane emission from rice soils based on cyanobacterial inoculants depends on the proper pairing of cyanobacteria–methanotrophs and their respective traits.

U2 - 10.3390/microorganisms11122830

DO - 10.3390/microorganisms11122830

M3 - Article

SN - 2076-2607

VL - 11

JO - Microorganisms

JF - Microorganisms

IS - 12

M1 - 2830

ER -

Interactions between Cyanobacteria and Methane Processing Microbes Mitigate Methane Emissions from Rice Soils

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