Documents

  • 5884_Philmus

    Final published version, 793 KB, PDF-document

    Request copy

DOI

  • Benjamin Philmus
  • Brenda Shaffer
  • Teresa Kidarsa
  • Qing Yan
  • Jos Raaijmakers
  • Tadhg Begley
  • Joyce Loper
Pseudomonas spp. are prolific producers of natural products from many structural classes. Here we show that the soil bacterium Pseudomonas protegens Pf-5 is capable of producing trace levels of the triazine natural product toxoflavin (1) under microaerobic conditions. We evaluated toxoflavin production by derivatives of Pf-5 having deletions in specific biosynthesis genes, which led us to propose a new biosynthetic pathway for toxoflavin that shares the first two steps with riboflavin biosynthesis. We also report that toxM, which is not present in the well-characterized cluster of Burkholderia glumae, encodes a monooxygenase that degrades toxoflavin. The toxoflavin degradation product of ToxM is identical to that of TflA, the toxoflavin lyase from Paenibacillus polymyxa. Toxoflavin production by P. protegens causes inhibition of several plant-pathogenic bacteria, and introduction of toxM into the toxoflavin-sensitive strain P. syringae DC3000 results in resistance to toxoflavin.
Original languageEnglish
Pages (from-to)1782-1790
JournalChemBioChem
Volume16
Issue number12
DOI
Publication statusPublished - 2015

    Research areas

  • triazine, secondary metabolism, biocontrol, international

ID: 1176764