Isolation and in vitro expansion of human colonic stem cells

P. Jung, T. Sato, A. Merlos-Suarez, F.M. Barriga, M. Iglesias, D. Rossell, H. Auer, M. Gallardo, M.A. Blasco, E. Sancho, H. Clevers, E. Batlle

Research output: Contribution to journal/periodicalArticleScientificpeer-review

502 Citations (Scopus)


Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population. [KEYWORDS: Cell Differentiation/physiology, Cell Proliferation, Colon/ cytology, Flow Cytometry, Humans, Immunohistochemistry, Intestinal Mucosa/ cytology, Microscopy, Confocal, Multipotent Stem Cells/cytology/ physiology, Real-Time Polymerase Chain Reaction, Receptor, EphB2/ metabolism, Telomere/metabolism]
Original languageEnglish
Pages (from-to)1225-1227
JournalNature Medicine
Issue number10
Publication statusPublished - 2011


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