Isolation of deletion alleles by G4 DNA-induced mutagenesis.

D.B. Pontier, E. Kruisselbrink, V. Guryev, M. Tijsterman

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G(3)N(x)G(3)N(x)G(3)N(x)G(3), a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes.
Original languageEnglish
Pages (from-to)655-657
JournalNature Methods
Volume6
Issue number9
DOIs
Publication statusPublished - 2009

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